Detail projektu

Unraveling the role of polyhydroxyalkanoates in Schlegelella thermodepolymerans – promising environmental bacterium for next generation biotechnology

Období řešení: 01.01.2022 — 31.12.2024

Zdroje financování

Grantová agentura České republiky - Standardní projekty

- plně financující (2022-04-26 - 2024-12-31)

O projektu

Schlegelella thermodepolymerans is a thermophilic bacterium that was isolated thanks to capability of polymer degradation. Nevertheless, we have recently identified the extraordinary biotechnological potential of the bacterium within the concept of Next Generation Biotechnology. Apart from polymer degradation, S. thermodepolymerans is also able to produce polyhydroxyalkanoates (PHA), microbial polyesters serving as a storage of carbon and energy and enhancing stress robustness of bacterial cells against numerous environmental stressors. Moreover, PHA are considered to be an ecological alternative to petrochemical polymers. In the proposed project we will in-depth look into PHA metabolism of S. thermodepolymerans especially in the context of stress robustness of the bacterium, we will construct a detailed metabolic model, develop and optimize molecular tools for genome editing and we will also test the employment of the bacterium in the most promising biotechnological applications - production of PHA, biodegradation of polymers and biotransformation/degradation of phenolic compounds.

Klíčová slova
Schlegelella thermodepolymerans; polyhydroxyalkanoates; environmental biotechnology; industrial biotechnology; biodegradation; bioinfromatics; genetic enineering

Označení

GA22-10845S

Originální jazyk

angličtina

Řešitelé

Obruča Stanislav, prof. Ing., Ph.D. - hlavní řešitel
Sedlář Karel, Mgr. Ing., Ph.D. - spoluřešitel

Útvary

Centrum materiálového výzkumu
- odpovědné pracoviště (07.04.2021 - 07.04.2021)
CMV - laboratoř biotechnologií
- odpovědné pracoviště (07.04.2021 - 07.04.2021)
Fakulta chemická
- odpovědné pracoviště (07.04.2021 - 07.04.2021)
Ústav chemie potravin a biotechnologií
- příjemce (01.01.2022 - 31.12.2024)
Ústav biomedicínského inženýrství
- spolupříjemce (01.01.2022 - 31.12.2024)

Výsledky

SEDLÁŘ, K.; MUSILOVÁ, J.; KOUŘILOVÁ, X.; BEZDÍČEK, M.; OBRUČA, S. Diversity of Schlegelella thermodepolymerans Revealed with Nanopore Sequencing. In Proceedings of the 9th International Conference on Chemical Technology. Prague: Czech Society of Industrial Chemistry, 2022. p. 373-378. ISBN: 978-80-88307-11-2.
Detail

MUSILOVÁ, J.; KOUŘILOVÁ, X.; BEZDÍČEK, M.; OBRUČA, S.; SEDLÁŘ, K. Comparison of short and long reads in structural and functional annotation of non-model bacteria. 2022. p. 11-11.
Detail

MUSILOVÁ, J.; KOUŘILOVÁ, X.; HEŘMÁNKOVÁ, K.; BEZDÍČEK, M.; OBRUČA, S.; SEDLÁŘ, K. Comparative genomic analysis of two Schlegelella species brings novel insights into polyhydroxyalkanoates production. 10th International Chemical-Technological Conference (ICCT), Mikulov, Czechia. 2023.
Detail

Odpovědnost: Obruča Stanislav, prof. Ing., Ph.D.