Using Of “Hot” Genus Aneurinibacillus To Produce Unique Polyhydroxyalkanoate copolymers

Using Of “Hot” Genus Aneurinibacillus To Produce Unique Polyhydroxyalkanoate copolymers

Abstrakt

Polyhydroxyalkanoates (PHA) are microbial polymers that serve as a source of carbon and energy for bacteria. Moreover, they are a suitable alternative to polymers produced from fossil resources. Although they have many advantages - biocompatibility, biodegradability, wide application use; their production costs are still high. Over 40 thermophilic strains were obtained using an original isolation protocol that uses changes in osmotic stress as a selection. After characterization, a large proportion of these strains were assigned to the previously poorly described Aneurinibacillus species. However, the genus Aneurinibacillus appears to be a very interesting PHA producer with great potential. Firstly, it can use waste glycerol as a carbon source, but also synthesizes interesting and unusual copolymers when suitable precursors are used. Isolates of this genus are able to incorporate PHA monomers such as 4-hydroxybutyrate in high fraction content (up to 90 mol.%) into their polymers. Also, with the use of a suitable precursor, 4-hydroxyvalerate, 5-hydroxyvalerate, but also monomers of mcl- PHA namely 3-hydroxyhexanoate or 4-hydroxyhexanoate can be incorporated into the PHA structure

Polyhydroxyalkanoates (PHA) are microbial polymers that serve as a source of carbon and energy for bacteria. Moreover, they are a suitable alternative to polymers produced from fossil resources. Although they have many advantages - biocompatibility, biodegradability, wide application use; their production costs are still high. Over 40 thermophilic strains were obtained using an original isolation protocol that uses changes in osmotic stress as a selection. After characterization, a large proportion of these strains were assigned to the previously poorly described Aneurinibacillus species. However, the genus Aneurinibacillus appears to be a very interesting PHA producer with great potential. Firstly, it can use waste glycerol as a carbon source, but also synthesizes interesting and unusual copolymers when suitable precursors are used. Isolates of this genus are able to incorporate PHA monomers such as 4-hydroxybutyrate in high fraction content (up to 90 mol.%) into their polymers. Also, with the use of a suitable precursor, 4-hydroxyvalerate, 5-hydroxyvalerate, but also monomers of mcl- PHA namely 3-hydroxyhexanoate or 4-hydroxyhexanoate can be incorporated into the PHA structure

polyhydroxyalkanoates, thermophiles, Aneurinibacillus, copolymers

polyhydroxyalkanoates, thermophiles, Aneurinibacillus, copolymers

PERNICOVÁ, I.; ŘEHÁKOVÁ, V.; KOUŘILOVÁ, X.; SEDLÁČEK, P.; OBRUČA, S.

13.09.2023

VUT

Brno

European Symposium on Biopolymers Book of Abstracts

109

109

1