Detail publikačního výsledku

Morphological Study of PHA Producing Bacteria

HRUBANOVÁ, K.; SIKOROVÁ, P.; MRÁZOVÁ, K.; NEBESÁŘOVÁ, J.; OBRUČA, S.; KRZYŽÁNEK, V.

Originální název

Morphological Study of PHA Producing Bacteria

Anglický název

Morphological Study of PHA Producing Bacteria

Druh

Stať ve sborníku v databázi WoS či Scopus

Originální abstrakt

Our comprehensive morphological study indicates that the method involving cryo-SEM imaging in combination with highpressure HPF freezing and perpendicular freeze-fracturing, is an excellent technique for analysing highly hydrated samples. Cells containing polymer particles, which are elastic even at very low temperatures, were visualized using this method. By TEM it is able to monitor the distribution of PHB granules inside the microbial cells. SBFSEM provides spatial information. A comparison of the morphology of the internal and external cell structure shows that the Cupriavidus necator H16 contains significant amounts of PHA granules, which are distributed throughout the whole cell volume .

Anglický abstrakt

Our comprehensive morphological study indicates that the method involving cryo-SEM imaging in combination with highpressure HPF freezing and perpendicular freeze-fracturing, is an excellent technique for analysing highly hydrated samples. Cells containing polymer particles, which are elastic even at very low temperatures, were visualized using this method. By TEM it is able to monitor the distribution of PHB granules inside the microbial cells. SBFSEM provides spatial information. A comparison of the morphology of the internal and external cell structure shows that the Cupriavidus necator H16 contains significant amounts of PHA granules, which are distributed throughout the whole cell volume .

Klíčová slova

polyhydroxyalkanoates; electron microscopy; Cupriavidus necator

Klíčová slova v angličtině

polyhydroxyalkanoates; electron microscopy; Cupriavidus necator

Autoři

HRUBANOVÁ, K.; SIKOROVÁ, P.; MRÁZOVÁ, K.; NEBESÁŘOVÁ, J.; OBRUČA, S.; KRZYŽÁNEK, V.

Rok RIV

2024

Vydáno

31.10.2023

Nakladatel

Oxford Academic

Místo

Oxford

Kniha

Mictoscopy and Microanalysis

ISSN

1435-8115

Periodikum

MICROSCOPY AND MICROANALYSIS

Svazek

29

Číslo

1

Stát

Spojené státy americké

Strany od

883

Strany do

884

Strany počet

2

URL

https://watermark.silverchair.com/ozad067.436.pdf?token=AQECAHi208BE49Ooan9kkhW_Ercy7Dm3ZL_9Cf3qfKAc485ysgAAA24wggNqBgkqhkiG9w0BBwagggNbMIIDVwIBADCCA1AGCSqGSIb3DQEHATAeBglghkgBZQMEAS4wEQQM6zvKSZpfNQkegG0tAgEQgIIDIdKmjtZo1JTIbtb6RFZkFuVEwyCH50hW3BGT1LvIQEWQLtR2vrZuSYNt0-RFn4X-My459ZXHgDgT3rXVyN2hr5IheLy7h3coAB9uWNHZyMpECgDPA-kncAOzVulTsU3Kjj0NO5WEeWIPn_AD_PzvxEI3tKdoFX0tkT_Mig2zipqdYSafxFl9M7Eo3l-paA0fN24q4yb7KIe_uNS4DIL9MK9qy-r74U0ObIq4W00RuFrCimenl2YzSQ9e8U42dFsaNmZ9C7mod63Zd98fCNbOydwTwq9_puW6rJVG2OY7jDWF

BibTex

@inproceedings{BUT187572,
  author="Kamila {Hrubanová} and Pavlína {Sikorová} and Kateřina {Mrázová} and Jana {Nebesářová} and Stanislav {Obruča} and Vladislav {Krzyžánek}",
  title="Morphological Study of PHA Producing Bacteria",
  booktitle="Mictoscopy and Microanalysis",
  year="2023",
  journal="MICROSCOPY AND MICROANALYSIS",
  volume="29",
  number="1",
  pages="883--884",
  publisher="Oxford Academic",
  address="Oxford",
  doi="10.1093/micmic/ozad067.436",
  issn="1431-9276",
  url="https://watermark.silverchair.com/ozad067.436.pdf?token=AQECAHi208BE49Ooan9kkhW_Ercy7Dm3ZL_9Cf3qfKAc485ysgAAA24wggNqBgkqhkiG9w0BBwagggNbMIIDVwIBADCCA1AGCSqGSIb3DQEHATAeBglghkgBZQMEAS4wEQQM6zvKSZpfNQkegG0tAgEQgIIDIdKmjtZo1JTIbtb6RFZkFuVEwyCH50hW3BGT1LvIQEWQLtR2vrZuSYNt0-RFn4X-My459ZXHgDgT3rXVyN2hr5IheLy7h3coAB9uWNHZyMpECgDPA-kncAOzVulTsU3Kjj0NO5WEeWIPn_AD_PzvxEI3tKdoFX0tkT_Mig2zipqdYSafxFl9M7Eo3l-paA0fN24q4yb7KIe_uNS4DIL9MK9qy-r74U0ObIq4W00RuFrCimenl2YzSQ9e8U42dFsaNmZ9C7mod63Zd98fCNbOydwTwq9_puW6rJVG2OY7jDWF"
}