Detail publikačního výsledku

Structural changes in metallothionein isoforms revealed by capillary electrophoresis and Brdicka reaction

VACULOVIČOVÁ, M.; HYNEK, D.; ŠKUTKOVÁ, H.; ADAM, V.; PROVAZNÍK, I.; KIZEK, R.

Originální název

Structural changes in metallothionein isoforms revealed by capillary electrophoresis and Brdicka reaction

Anglický název

Structural changes in metallothionein isoforms revealed by capillary electrophoresis and Brdicka reaction

Druh

Článek WoS

Originální abstrakt

Metallothionein (MT) as a potential cancer marker is at the center of interest and its properties, function and behaviour under various condititons is intensively studied. In the present study, two major mammalian MT isoforms (MT-1 and MT-2) were separated using capillary electrophoresis (CE) coupled with UV detector in order to describe their basic behavior. The results were confirmed by manual spectrometric measurements and by different pulse voltammetry Brdicka reaction. Based on these results, a model of MT behaviour under the conditions studied was suggested.

Anglický abstrakt

Metallothionein (MT) as a potential cancer marker is at the center of interest and its properties, function and behaviour under various condititons is intensively studied. In the present study, two major mammalian MT isoforms (MT-1 and MT-2) were separated using capillary electrophoresis (CE) coupled with UV detector in order to describe their basic behavior. The results were confirmed by manual spectrometric measurements and by different pulse voltammetry Brdicka reaction. Based on these results, a model of MT behaviour under the conditions studied was suggested.

Klíčová slova

Brdicka reaction; Cancer; CE; Denaturation; Electrochemistry; Isoforms; Metallothionein

Klíčová slova v angličtině

Brdicka reaction; Cancer; CE; Denaturation; Electrochemistry; Isoforms; Metallothionein

Autoři

VACULOVIČOVÁ, M.; HYNEK, D.; ŠKUTKOVÁ, H.; ADAM, V.; PROVAZNÍK, I.; KIZEK, R.

Rok RIV

2013

Vydáno

01.01.2012

ISSN

0173-0835

Periodikum

ELECTROPHORESIS

Svazek

33

Číslo

2

Stát

Spolková republika Německo

Strany od

270

Strany do

279

Strany počet

10

BibTex

@article{BUT90347,
  author="Markéta {Vaculovičová} and David {Hynek} and Helena {Vítková} and Vojtěch {Adam} and Valentýna {Provazník} and René {Kizek}",
  title="Structural changes in metallothionein isoforms revealed by capillary electrophoresis and Brdicka reaction",
  journal="ELECTROPHORESIS",
  year="2012",
  volume="33",
  number="2",
  pages="270--279",
  doi="10.1002/elps.201100312",
  issn="0173-0835"
}