Publication result detail

Structural changes in metallothionein isoforms revealed by capillary electrophoresis and Brdicka reaction

VACULOVIČOVÁ, M.; HYNEK, D.; ŠKUTKOVÁ, H.; ADAM, V.; PROVAZNÍK, I.; KIZEK, R.

Original Title

Structural changes in metallothionein isoforms revealed by capillary electrophoresis and Brdicka reaction

English Title

Structural changes in metallothionein isoforms revealed by capillary electrophoresis and Brdicka reaction

Type

WoS Article

Original Abstract

Metallothionein (MT) as a potential cancer marker is at the center of interest and its properties, function and behaviour under various condititons is intensively studied. In the present study, two major mammalian MT isoforms (MT-1 and MT-2) were separated using capillary electrophoresis (CE) coupled with UV detector in order to describe their basic behavior. The results were confirmed by manual spectrometric measurements and by different pulse voltammetry Brdicka reaction. Based on these results, a model of MT behaviour under the conditions studied was suggested.

English abstract

Metallothionein (MT) as a potential cancer marker is at the center of interest and its properties, function and behaviour under various condititons is intensively studied. In the present study, two major mammalian MT isoforms (MT-1 and MT-2) were separated using capillary electrophoresis (CE) coupled with UV detector in order to describe their basic behavior. The results were confirmed by manual spectrometric measurements and by different pulse voltammetry Brdicka reaction. Based on these results, a model of MT behaviour under the conditions studied was suggested.

Keywords

Brdicka reaction; Cancer; CE; Denaturation; Electrochemistry; Isoforms; Metallothionein

Key words in English

Brdicka reaction; Cancer; CE; Denaturation; Electrochemistry; Isoforms; Metallothionein

Authors

VACULOVIČOVÁ, M.; HYNEK, D.; ŠKUTKOVÁ, H.; ADAM, V.; PROVAZNÍK, I.; KIZEK, R.

RIV year

2013

Released

01.01.2012

ISBN

0173-0835

Periodical

ELECTROPHORESIS

Volume

33

Number

2

State

Federal Republic of Germany

Pages from

270

Pages to

279

Pages count

10

BibTex

@article{BUT90347,
  author="Markéta {Vaculovičová} and David {Hynek} and Helena {Vítková} and Vojtěch {Adam} and Valentýna {Provazník} and René {Kizek}",
  title="Structural changes in metallothionein isoforms revealed by capillary electrophoresis and Brdicka reaction",
  journal="ELECTROPHORESIS",
  year="2012",
  volume="33",
  number="2",
  pages="270--279",
  doi="10.1002/elps.201100312",
  issn="0173-0835"
}