Effects of Voltage Sensitive Dye di-4-ANEPPS on Guinea Pig Myocardium

Effects of Voltage Sensitive Dye di-4-ANEPPS on Guinea Pig Myocardium

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Voltage-sensitive dyes have been employed to record monophasic action potentials (MAPs) in a variety of heart preparations with results comparable to classical microelectrode method. However, still little data are available on the response of the heart muscle to the loading with the dye. One of the most consistently used dyes – di-4-ANEPPS - is employed in our laboratory to record MAPs from isolated guinea pig hearts. It is of great importance to prove that loading of the heart muscle does not result in pharmacological or toxic effects to the preparation, since the experiments are of long duration, e.g. to prove the viability and quality of the heart at the beginning of the experiment.

Voltage-sensitive dyes have been employed to record monophasic action potentials (MAPs) in a variety of heart preparations with results comparable to classical microelectrode method. However, still little data are available on the response of the heart muscle to the loading with the dye. One of the most consistently used dyes – di-4-ANEPPS - is employed in our laboratory to record MAPs from isolated guinea pig hearts. It is of great importance to prove that loading of the heart muscle does not result in pharmacological or toxic effects to the preparation, since the experiments are of long duration, e.g. to prove the viability and quality of the heart at the beginning of the experiment.

Voltage-sensitive dye, monophasic action potential, heart, di-4-ANEPPS

Voltage-sensitive dye, monophasic action potential, heart, di-4-ANEPPS

Nováková M., Provazník I., Bardoňová J., Blaha M.

05.09.2005

IFBME

Praha

0-7803-8894-1

Proceedings of EMBEC 2005

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