Detail publikačního výsledku

Experimental methods for simultaneous measurement of action potentials and electrograms in isolated heart

KOLÁŘOVÁ, J.; FIALOVÁ, K.; JANOUŠEK, O.; NOVÁKOVÁ, M.; PROVAZNÍK, I.

Originální název

Experimental methods for simultaneous measurement of action potentials and electrograms in isolated heart

Anglický název

Experimental methods for simultaneous measurement of action potentials and electrograms in isolated heart

Druh

Článek WoS

Originální abstrakt

Monophasic action potential (MAP) can be recorded from the heart surface by optical method based on fluorescence measurement. The motion of isolated heart during experiment caused additional noise in recorded signal. The motion artifact can be eliminated by ratiometric fluorescence emission measurements. This study is based on experiments in which optical MAP measurement is done by single-wavelength and dual-wavelength measurement of fluorescence emission. Both recording setups are presented and their advantages and disadvantages are discussed. MAPs recorded by both methods from isolated rabbit hearts perfused according to Langendorff are presented. Simultaneous electrograms (EG) and MAPs recording are analyzed and measurement of velocity of impulse conduction through heart tissue is presented.

Anglický abstrakt

Monophasic action potential (MAP) can be recorded from the heart surface by optical method based on fluorescence measurement. The motion of isolated heart during experiment caused additional noise in recorded signal. The motion artifact can be eliminated by ratiometric fluorescence emission measurements. This study is based on experiments in which optical MAP measurement is done by single-wavelength and dual-wavelength measurement of fluorescence emission. Both recording setups are presented and their advantages and disadvantages are discussed. MAPs recorded by both methods from isolated rabbit hearts perfused according to Langendorff are presented. Simultaneous electrograms (EG) and MAPs recording are analyzed and measurement of velocity of impulse conduction through heart tissue is presented.

Klíčová slova

isolated heart, action potential, electrogram, voltage sensitive dye, ratiometry

Klíčová slova v angličtině

isolated heart, action potential, electrogram, voltage sensitive dye, ratiometry

Autoři

KOLÁŘOVÁ, J.; FIALOVÁ, K.; JANOUŠEK, O.; NOVÁKOVÁ, M.; PROVAZNÍK, I.

Rok RIV

2011

Vydáno

28.05.2010

Nakladatel

AV ČR

Místo

Praha

ISSN

0862-8408

Periodikum

PHYSIOLOGICAL RESEARCH

Svazek

59

Číslo

Suppl 1

Stát

Česká republika

Strany od

S71

Strany do

S80

Strany počet

10

Plný text v Digitální knihovně

http://hdl.handle.net/