Detail publikačního výsledku

Comparison of Two Procedures of Loading with Voltage-Sensitive Dye Di-4-ANEPPS in Rabbit Isolated Heart

NOVÁKOVÁ, M.; NOGOVÁ, K.; BARDOŇOVÁ, J.; PROVAZNÍK, I.

Originální název

Comparison of Two Procedures of Loading with Voltage-Sensitive Dye Di-4-ANEPPS in Rabbit Isolated Heart

Anglický název

Comparison of Two Procedures of Loading with Voltage-Sensitive Dye Di-4-ANEPPS in Rabbit Isolated Heart

Druh

Stať ve sborníku v databázi WoS či Scopus

Originální abstrakt

Recording of monophasic action potentials (MAPs) by optical method using voltage sensitive dyes has been established as possibility to measure membrane potential changes in certain models. We studied two procedures of isolated rabbit hearts loading with di-4-ANEPPS - "slow" method (perfusion with low concentration of dye for longer time) or "fast" method (single microinjection of highly concentrated bolus of the dye in the aorta). Signal-to-noise ratio was evaluated in each minute of continuous exposure to excitatory light and persistence of dye in the tissue was estimated. We conclude that both methods enable us to record MAPs, but during long exposure to light the persistence of the dye in the tissue is insufficient in "fast" method. Although the "slow" loading affects electrophysiological properties of the heart, in case of rabbit heart it is negligible since its relative resistance to the side-effects of di-4-ANEPPS.

Anglický abstrakt

Recording of monophasic action potentials (MAPs) by optical method using voltage sensitive dyes has been established as possibility to measure membrane potential changes in certain models. We studied two procedures of isolated rabbit hearts loading with di-4-ANEPPS - "slow" method (perfusion with low concentration of dye for longer time) or "fast" method (single microinjection of highly concentrated bolus of the dye in the aorta). Signal-to-noise ratio was evaluated in each minute of continuous exposure to excitatory light and persistence of dye in the tissue was estimated. We conclude that both methods enable us to record MAPs, but during long exposure to light the persistence of the dye in the tissue is insufficient in "fast" method. Although the "slow" loading affects electrophysiological properties of the heart, in case of rabbit heart it is negligible since its relative resistance to the side-effects of di-4-ANEPPS.

Klíčová slova

Recording, Voltage-Sensitive Dye, Action Potentials

Klíčová slova v angličtině

Recording, Voltage-Sensitive Dye, Action Potentials

Autoři

NOVÁKOVÁ, M.; NOGOVÁ, K.; BARDOŇOVÁ, J.; PROVAZNÍK, I.

Rok RIV

2010

Vydáno

01.12.2008

Nakladatel

IEEE

Místo

Bologna, Italy

ISBN

978-1-4244-3706-1

Kniha

Computers in Cardiology

ISSN

0276-6574

Periodikum

Computers in Cardiology

Stát

Spojené státy americké

Strany od

1

Strany do

4

Strany počet

4

Plný text v Digitální knihovně

http://hdl.handle.net/

BibTex

@inproceedings{BUT27047,
  author="Marie {Nováková} and Kateřina {Nogová} and Jana {Kolářová} and Valentýna {Provazník}",
  title="Comparison of Two Procedures of Loading with Voltage-Sensitive Dye Di-4-ANEPPS in Rabbit Isolated Heart",
  booktitle="Computers in Cardiology",
  year="2008",
  journal="Computers in Cardiology",
  pages="1--4",
  publisher="IEEE",
  address="Bologna, Italy",
  isbn="978-1-4244-3706-1",
  issn="0276-6574"
}