Detail publikace

Pseudomonas petrae sp. nov. isolated from regolith samples in Antarctica

NOVÁKOVÁ, D. KOUBLOVÁ, V. SEDLÁŘ, K. STAŇKOVÁ, E. KRÁLOVÁ, S. ŠVEC, P. NEUMANN-SCHAAL, M. WOLF, J. KOUDELKOVÁ, S. BARTÁK, M. SEDLÁČEK, I.

Originální název

Pseudomonas petrae sp. nov. isolated from regolith samples in Antarctica

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

A polyphasic taxonomic approach was used to characterize the four strains P2653T, P2652, P2498, and P2647, isolated from Antarctic regolith samples. Initial genotype screening performed by PCR fingerprinting based on repetitive sequences showed that the isolates studied formed a coherent cluster separated from the other Pseudomonas species. Identification results based on 16S rRNA gene sequences showed the highest sequence similarity with Pseudomonas graminis (99.7%), which was confirmed by multilocus sequence analysis using the rpoB, rpoD, and gyrB genes. Genome sequence comparison of P2653T with the most related P. graminis type strain DSM 11363T revealed an average nucleotide identity of 92.1% and a digital DNA-DNA hybridization value of 46.6%. The major fatty acids for all Antarctic strains were C16:0, Summed Feature 3 (C16:1 omega 7c/C16:1 omega 6c) and Summed Feature 8 (C18:1 omega 7c/C18:1 omega 6c). The predominant respiratory quinone was Q-9, and the major polar lipids were phosphatidylethanolamine, diphosphatidylglycerol, and phosphatidylglycerol. The regolith strains could be differentiated from related species by the absence of arginine dihydrolase, ornithine and lysine decarboxylase and by negative tyrosine hydrolysis. The results of this polyphasic study allowed the genotypic and phenotypic differentiation of four analysed strains from the closest related species, which confirmed that the strains represent a novel species within the genus Pseudomonas, for which the name Pseudomonas petrae sp. nov. is proposed with P2653T (CCM 8850T = DSM 112068T = LMG 30619T) as the type strain.

Klíčová slova

Pseudomonas; P. petrae sp. nov; Antarctica; Regolith; Species description; Taxonomy

Autoři

NOVÁKOVÁ, D.; KOUBLOVÁ, V.; SEDLÁŘ, K.; STAŇKOVÁ, E.; KRÁLOVÁ, S.; ŠVEC, P.; NEUMANN-SCHAAL, M.; WOLF, J.; KOUDELKOVÁ, S.; BARTÁK, M.; SEDLÁČEK, I.

Vydáno

9. 5. 2023

Nakladatel

ELSEVIER GMBH

Místo

MUNICH

ISSN

0723-2020

Periodikum

SYSTEMATIC AND APPLIED MICROBIOLOGY

Ročník

46

Číslo

4

Stát

Spolková republika Německo

Strany od

1

Strany do

9

Strany počet

9

URL

https://www.sciencedirect.com/science/article/pii/S0723202023000334

BibTex

@article{BUT183881,
  author="Dana {Nováková} and Vendula {Koublová} and Karel {Sedlář} and Eva {Staňková} and Stanislava {Králová} and Pavel {Švec} and Meina {Neumann-Schaal} and Jacqueline {Wolf} and Sylva {Koudelková} and Miloš {Barták} and Ivo {Sedláček}",
  title="Pseudomonas petrae sp. nov. isolated from regolith samples in Antarctica",
  journal="SYSTEMATIC AND APPLIED MICROBIOLOGY",
  year="2023",
  volume="46",
  number="4",
  pages="1--9",
  doi="10.1016/j.syapm.2023.126424",
  issn="0723-2020",
  url="https://www.sciencedirect.com/science/article/pii/S0723202023000334"
}