Detail publikace

DUCT reveals architectural mechanisms contributing to bile duct recovery in a mouse model for Alagille syndrome

HANKEOVÁ, S. ŠALPLACHTA, J. ZIKMUND, T. KAVKOVÁ, M. VAN HUL, N. BŘÍNEK, A. SMÉKALOVÁ, V. LÁZŇOVSKÝ, J. DAWIT, F. JAROŠ, J. BRYJA, V. LENDAHL, U. ELLIS, E. NEMETH, A. FISCHLER, B. HANNESO, E. KAISER, J. ANDERSSON, E.

Originální název

DUCT reveals architectural mechanisms contributing to bile duct recovery in a mouse model for Alagille syndrome

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Organ function depends on tissues adopting the correct architecture. However, insights into organ architecture are currently hampered by an absence of standardized quantitative 3D analysis. We aimed to develop a robust technology to visualize, digitalize, and segment the architecture of two tubular systems in 3D: double resin casting micro computed tomography (DUCT). As proof of principle, we applied DUCT to a mouse model for Alagille syndrome (Jag1Ndr/Ndr mice), characterized by intrahepatic bile duct paucity, that can spontaneously generate a biliary system in adulthood. DUCT identified increased central biliary branching and peripheral bile duct tortuosity as two compensatory processes occurring in distinct regions of Jag1Ndr/Ndr liver, leading to full reconstitution of wild-type biliary volume and phenotypic recovery. DUCT is thus a powerful new technology for 3D analysis, which can reveal novel phenotypes and provide a standardized method of defining liver architecture in mouse models.

Klíčová slova

Alagille syndrome; MicroCT; cholangiopathy; human; mouse; physics of living systems; regenerative medicine; resin; stem cells; vasculature.

Autoři

HANKEOVÁ, S.; ŠALPLACHTA, J.; ZIKMUND, T.; KAVKOVÁ, M.; VAN HUL, N.; BŘÍNEK, A.; SMÉKALOVÁ, V.; LÁZŇOVSKÝ, J.; DAWIT, F.; JAROŠ, J.; BRYJA, V.; LENDAHL, U.; ELLIS, E.; NEMETH, A.; FISCHLER, B.; HANNESO, E.; KAISER, J.; ANDERSSON, E.

Vydáno

26. 2. 2021

Nakladatel

ELIFE SCIENCES PUBLICATIONS LTD

Místo

Cambridge

ISSN

2050-084X

Periodikum

eLife

Číslo

10

Stát

Spojené království Velké Británie a Severního Irska

Strany od

1

Strany do

29

Strany počet

29

URL

https://elifesciences.org/articles/60916#content

Plný text v Digitální knihovně

http://hdl.handle.net/11012/201001

BibTex

@article{BUT170436,
  author="Simona {Hankeová} and Jakub {Šalplachta} and Tomáš {Zikmund} and Michaela {Kavková} and Noemi {Van Hul} and Adam {Břínek} and Veronika {Smékalová} and Jakub {Lázňovský} and Feven {Dawit} and Josef {Jaroš} and Vítězslav {Bryja} and Urban {Lendahl} and ewa {ellis} and Antal {Nemeth} and Björn {Fischler} and Edouard {Hanneso} and Jozef {Kaiser} and Emma Rachel {Andersson}",
  title="DUCT reveals architectural mechanisms contributing to bile duct recovery in a mouse model for Alagille syndrome",
  journal="eLife",
  year="2021",
  number="10",
  pages="1--29",
  doi="10.7554/eLife.60916",
  issn="2050-084X",
  url="https://elifesciences.org/articles/60916#content"
}