Detail publikace

Cell death type identification by quantitative phase imaging

KŘÍŽOVÁ, A. BALVAN, J. GUMULEC, J. SLABÝ, T. MASAŘÍK, M. CHMELÍK, R.

Originální název

Cell death type identification by quantitative phase imaging

Typ

abstrakt

Jazyk

angličtina

Originální abstrakt

Exact identification of cell death type is important for instance in diagnostics, dose-response, and toxicological studies. Predominant types of cell death are usually detected by flow-cytometry (FCM), where the absence of the analysis of cellular morphology leads to the misclassification of cell death type due to underestimated oncosis. The definition of oncosis is important because of its potential reversibility. Therefore, FCM analysis of cell death using the annexin V/propidium iodide assay was compared with quantitative phase imaging (QPI) coupled with fluorescence detection.

Klíčová slova

Cell death type identification

Autoři

KŘÍŽOVÁ, A.; BALVAN, J.; GUMULEC, J.; SLABÝ, T.; MASAŘÍK, M.; CHMELÍK, R.

Vydáno

15. 4. 2015

ISSN

1059-1524

Periodikum

MOLECULAR BIOLOGY OF THE CELL

Ročník

26

Stát

Spojené státy americké

Strany od

P1553

Strany počet

1