Detail publikačního výsledku

Impedimetric Aptasensor for Thrombin Recognition Based on CD Support

CASTILLO, G.; TRNKOVÁ, L.; HRDÝ, R.; HIANIK, T.

Originální název

Impedimetric Aptasensor for Thrombin Recognition Based on CD Support

Anglický název

Impedimetric Aptasensor for Thrombin Recognition Based on CD Support

Druh

Článek WoS

Originální abstrakt

We report an aptamer biosensing array for thrombin detection by measuring the electrochemical impedance upon aptamer-protein formation at the surface of CD-trodes (GCDTs) in the presence of the redox couple [Fe(CN)6]3/4. GCDTs are fabricated from recordable compact discs that contain a fine gold layer. The biosensor is constructed by self-assembling of a thiol-modified thrombin binding aptamer (TBA) onto a GCDT surface. The sensor reveals good ligand specificity, recognition in a wide range of thrombin concentrations from 20 nM to 1 mM with a limit of detection of 5 nM

Anglický abstrakt

We report an aptamer biosensing array for thrombin detection by measuring the electrochemical impedance upon aptamer-protein formation at the surface of CD-trodes (GCDTs) in the presence of the redox couple [Fe(CN)6]3/4. GCDTs are fabricated from recordable compact discs that contain a fine gold layer. The biosensor is constructed by self-assembling of a thiol-modified thrombin binding aptamer (TBA) onto a GCDT surface. The sensor reveals good ligand specificity, recognition in a wide range of thrombin concentrations from 20 nM to 1 mM with a limit of detection of 5 nM

Klíčová slova

DNA aptamers, Biosensors, Human thrombin, Electrochemical impedance spectroscopy

Klíčová slova v angličtině

DNA aptamers, Biosensors, Human thrombin, Electrochemical impedance spectroscopy

Autoři

CASTILLO, G.; TRNKOVÁ, L.; HRDÝ, R.; HIANIK, T.

Rok RIV

2013

Vydáno

01.05.2012

ISSN

1040-0397

Periodikum

ELECTROANALYSIS

Svazek

24

Číslo

5

Stát

Spolková republika Německo

Strany od

1079

Strany do

1087

Strany počet

9

BibTex

@article{BUT92982,
  author="Gabriela {Castillo} and Libuše {Trnková} and Radim {Hrdý} and Tibor {Hianik}",
  title="Impedimetric Aptasensor for Thrombin Recognition Based on CD Support",
  journal="ELECTROANALYSIS",
  year="2012",
  volume="24",
  number="5",
  pages="1079--1087",
  doi="10.1002/elan.201200041",
  issn="1040-0397"
}