Detail publikačního výsledku

Parallel-Mode Confocal Microscopy

CHMELÍK, R., HARNA, Z.

Originální název

Parallel-Mode Confocal Microscopy

Anglický název

Parallel-Mode Confocal Microscopy

Druh

Článek recenzovaný mimo WoS a Scopus

Originální abstrakt

Conventional confocal microscopes are based on the dual scanning of a specimen by the images of a point source and of a point detector. Hence, their imaging mode is serial, i.e., the confocal image is obtained from the sequence of single-point or multiple-point partial images. Parallel-mode confocal imaging is possible on the basis of broad-source image-plane holography. We adapted this classical-holography technique for real-time reflected-light microscopy. The imaging speed of the parallel-mode confocal microscope is not limited by any part of the optical system, but only by the image detection and storage systems. Both the image phase and the image amplitude are reconstructed from the interference signal. It was verified both theoretically and experimentally that the main imaging parameters of the microscope are comparable with those of a conventional confocal microscope. The only exception is the imaging speed, which can be much higher.

Anglický abstrakt

Conventional confocal microscopes are based on the dual scanning of a specimen by the images of a point source and of a point detector. Hence, their imaging mode is serial, i.e., the confocal image is obtained from the sequence of single-point or multiple-point partial images. Parallel-mode confocal imaging is possible on the basis of broad-source image-plane holography. We adapted this classical-holography technique for real-time reflected-light microscopy. The imaging speed of the parallel-mode confocal microscope is not limited by any part of the optical system, but only by the image detection and storage systems. Both the image phase and the image amplitude are reconstructed from the interference signal. It was verified both theoretically and experimentally that the main imaging parameters of the microscope are comparable with those of a conventional confocal microscope. The only exception is the imaging speed, which can be much higher.

Klíčová slova

Confocal microscopy, Holographic applications, Real-time imaging.

Klíčová slova v angličtině

Confocal microscopy, Holographic applications, Real-time imaging.

Autoři

CHMELÍK, R., HARNA, Z.

Vydáno

01.01.1999

ISSN

0091-3286

Periodikum

OPTICAL ENGINEERING

Svazek

38

Číslo

10

Stát

Spojené státy americké

Strany od

1635

Strany počet

5

BibTex

@article{BUT39686,
  author="Radim {Chmelík} and Zdeněk {Harna}",
  title="Parallel-Mode Confocal Microscopy",
  journal="OPTICAL ENGINEERING",
  year="1999",
  volume="38",
  number="10",
  pages="5",
  issn="0091-3286"
}