Transcriptomic insights into acid crash and non-sporulating cultivations of Clostridium beijerinckii NRRL B-598

Transcriptomic insights into acid crash and non-sporulating cultivations of Clostridium beijerinckii NRRL B-598

Abstrakt

Various bacterial strains are studied for their remarkable ability to thrive in diverse conditions and produce substances with great biotechnological potential. One such strain is Clostridium beijerinckii NRRL B-598, known for its ability to produce hydrogen and butanol during acetone-butanol-ethanol (ABE) fermentation. To better understand the ABE fermentation pathway at the gene level, several RNA-Seq datasets have been published, accompanied by transcriptomic studies focusing on standard and butanol-shocked cultivations. Here, we present two new RNA-Seq datasets: the first was obtained from a cultivation in which an acid crash phenomenon was observed, while the second was acquired from a non-sporulating cultivation. Both datasets consist of six time-points selected to capture key stages of ABE fermentation and enable direct comparison with data from standard cultivation. This enabled us to perform differential expression analysis and gene ontology enrichment analysis, identifying changes in expression profiles of genes related to the ABE fermentation pathway. Additionally, we uncovered significantly enriched molecular functions and biological processes associated with the acid crash event and the non-sporulating culture. Our findings provide new insights into the regulatory mechanisms of Clostridium beijerinckii NRRL B-598, contributing to a deeper understanding of its metabolic adaptation and biotechnological potential.

Various bacterial strains are studied for their remarkable ability to thrive in diverse conditions and produce substances with great biotechnological potential. One such strain is Clostridium beijerinckii NRRL B-598, known for its ability to produce hydrogen and butanol during acetone-butanol-ethanol (ABE) fermentation. To better understand the ABE fermentation pathway at the gene level, several RNA-Seq datasets have been published, accompanied by transcriptomic studies focusing on standard and butanol-shocked cultivations. Here, we present two new RNA-Seq datasets: the first was obtained from a cultivation in which an acid crash phenomenon was observed, while the second was acquired from a non-sporulating cultivation. Both datasets consist of six time-points selected to capture key stages of ABE fermentation and enable direct comparison with data from standard cultivation. This enabled us to perform differential expression analysis and gene ontology enrichment analysis, identifying changes in expression profiles of genes related to the ABE fermentation pathway. Additionally, we uncovered significantly enriched molecular functions and biological processes associated with the acid crash event and the non-sporulating culture. Our findings provide new insights into the regulatory mechanisms of Clostridium beijerinckii NRRL B-598, contributing to a deeper understanding of its metabolic adaptation and biotechnological potential.

ŠABATOVÁ, K.; BRANSKÁ, B.; PATÁKOVÁ, P.; SEDLÁŘ, K.

14.04.2025

978-80-88307-24-2

12th International Conference on Chemical Technology

158

158

1