Pigment Formation by Monascus pilosus DBM 4361 in Submerged Liquid Culture

Pigment Formation by Monascus pilosus DBM 4361 in Submerged Liquid Culture

Článek WoS

Monascus pilosus is usually cultivated on rice because of monacolin K. We focused on pigment production in submerged liquid culture (SLC) where M. pilosus produced different pigments compared to M. purpureus and M. ruber. From the group of classic Monascus pigments, there were formed mostly compounds with a five-carbon side chain, and the dominant pigment was monascuspiloin, a yellow pigment structurally similar to monascin. In SLC, previously undescribed patterns affecting pigment formation were observed, such as the Crabtree effect, carbon catabolite repression of pigments caused by glucose and other mono-/disaccharides, as well as nitrogen regulation, particularly repression of pigment formation by ammonium sulfate. The highest pigment concentration in the extract was obtained using an organic nitrogen source, specifically 340 mg/L for yellow pigments utilizing a combination of sucrose and tryptone, 346 mg/L for orange pigments using starch and tryptone, and 75 mg/L for red pigments using lactose and tryptone.

Monascus pilosus is usually cultivated on rice because of monacolin K. We focused on pigment production in submerged liquid culture (SLC) where M. pilosus produced different pigments compared to M. purpureus and M. ruber. From the group of classic Monascus pigments, there were formed mostly compounds with a five-carbon side chain, and the dominant pigment was monascuspiloin, a yellow pigment structurally similar to monascin. In SLC, previously undescribed patterns affecting pigment formation were observed, such as the Crabtree effect, carbon catabolite repression of pigments caused by glucose and other mono-/disaccharides, as well as nitrogen regulation, particularly repression of pigment formation by ammonium sulfate. The highest pigment concentration in the extract was obtained using an organic nitrogen source, specifically 340 mg/L for yellow pigments utilizing a combination of sucrose and tryptone, 346 mg/L for orange pigments using starch and tryptone, and 75 mg/L for red pigments using lactose and tryptone.

Monascus pilosus, pigments, catabolic repression, stress conditions

Monascus pilosus, pigments, catabolic repression, stress conditions

HUSAKOVA, M.; BEZDICEK, M.; BRANSKA, B.; SEDLÁŘ, K.; PATAKOVA, P.

08.10.2025

Journal of Agricultural and Food Chemistry

73

42

Spojené státy americké

26900

26909

10

https://pubs.acs.org/doi/10.1021/acs.jafc.5c08401

http://hdl.handle.net/11012/255605

pigment-formation-by-monascus-pilosus-dbm-4361-in-submerged-liquid-culture
pigment-formation-by-monascus-pilosus