Diagnostic Performance of an Automated Robot for MALDI Target Preparation in Microbial Identification

Diagnostic Performance of an Automated Robot for MALDI Target Preparation in Microbial Identification

Článek WoS

The MBT Pathfinder® is an automated colony-picking robot designed for efficient sample preparation in matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. This article presents results from three key experiments evaluating the instrument's performance in conjunction with MALDI Biotyper® instrument. The method comparison experiment assessed its clinical performance, demonstrating comparable results with Gram-positive, Gram-negative, and anaerobic bacteria (scores larger than 2.00) and superior performance over simple direct yeast transfer (score: 1.80) when compared to samples prepared manually. The repeatability experiment confirmed consistent performance over multiple days and labs (average log score: 2.12, std. deviation: 0.59). The challenge panel experiment showcased its consistent and accurate performance across various samples and settings, yielding average scores between 1.76 and 2.19. These findings underline the MBT Pathfinder® as a reliable and efficient tool for MALDI-TOF mass spectrometry sample preparation in clinical and research applications.

The MBT Pathfinder® is an automated colony-picking robot designed for efficient sample preparation in matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. This article presents results from three key experiments evaluating the instrument's performance in conjunction with MALDI Biotyper® instrument. The method comparison experiment assessed its clinical performance, demonstrating comparable results with Gram-positive, Gram-negative, and anaerobic bacteria (scores larger than 2.00) and superior performance over simple direct yeast transfer (score: 1.80) when compared to samples prepared manually. The repeatability experiment confirmed consistent performance over multiple days and labs (average log score: 2.12, std. deviation: 0.59). The challenge panel experiment showcased its consistent and accurate performance across various samples and settings, yielding average scores between 1.76 and 2.19. These findings underline the MBT Pathfinder® as a reliable and efficient tool for MALDI-TOF mass spectrometry sample preparation in clinical and research applications.

agar plates, synthetic image generation, deep learning, semantic segmentation

agar plates, synthetic image generation, deep learning, semantic segmentation

PRANADA, A.; ČIČATKA, M.; KARÁSEK, J.; HEß, C.

2025

19.09.2024

American Society for Microbiology

0095-1137

JOURNAL OF CLINICAL MICROBIOLOGY

09-2024

Spojené státy americké

1

12

12

https://journals.asm.org/doi/10.1128/jcm.00434-24

http://hdl.handle.net/11012/249488

pranada-et-al-2024-diagnostic-performance-of-an-automated-robot-for-maldi-target-preparation-in-microbial-identification