Detail publikačního výsledku

Smallest dual-color qPCR device

Hanliang Zhu; Xiaocheng Liu; Yingqi Wang; Antao Sun; Tomas Teply; Marie Korabecna; Haoqing Zhang; Pavel Neuzil

Originální název

Smallest dual-color qPCR device

Anglický název

Smallest dual-color qPCR device

Druh

Článek WoS

Originální abstrakt

The global outbreak of the coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), necessitated the development of point-of-care (POC) systems for rapid and widespread population screening. Current POC systems, based on reverse transcription–quantitative polymerase chain reaction (RT-qPCR) methods, are unable to perform RNA target multiplexing, which is crucial for specific viral strain identification. This study presents a compact qPCR system capable of dual fluorescence channel gene multiplexing by simultaneously detecting two targets of the SARS-CoV-2 virus using 6-carboxyfluorescein (FAM)-and TxRed-labeled probes. The device measures (110 × 83 × 41) mm3 and weighs 210 gm. It features four virtual reaction chambers, a thermal cycle system, a real-time optical signal detection module, a USB interface, and a Bluetooth wireless data transmission module. Moreover, it can perform single fluorescent channel detection of PCR using intercalating dye, followed by a melting curve analysis and loop-mediated isothermal amplification. The proposed compact dual-color qPCR device significantly broadens its potential for POC applications, such as diagnostics or field testing, molecular biology, microfluidics, and virus detection.

Anglický abstrakt

The global outbreak of the coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), necessitated the development of point-of-care (POC) systems for rapid and widespread population screening. Current POC systems, based on reverse transcription–quantitative polymerase chain reaction (RT-qPCR) methods, are unable to perform RNA target multiplexing, which is crucial for specific viral strain identification. This study presents a compact qPCR system capable of dual fluorescence channel gene multiplexing by simultaneously detecting two targets of the SARS-CoV-2 virus using 6-carboxyfluorescein (FAM)-and TxRed-labeled probes. The device measures (110 × 83 × 41) mm3 and weighs 210 gm. It features four virtual reaction chambers, a thermal cycle system, a real-time optical signal detection module, a USB interface, and a Bluetooth wireless data transmission module. Moreover, it can perform single fluorescent channel detection of PCR using intercalating dye, followed by a melting curve analysis and loop-mediated isothermal amplification. The proposed compact dual-color qPCR device significantly broadens its potential for POC applications, such as diagnostics or field testing, molecular biology, microfluidics, and virus detection.

Klíčová slova

Miniaturization/integration; Multiplexing; RT-qPCR; Virus detection

Klíčová slova v angličtině

Miniaturization/integration; Multiplexing; RT-qPCR; Virus detection

Autoři

Hanliang Zhu; Xiaocheng Liu; Yingqi Wang; Antao Sun; Tomas Teply; Marie Korabecna; Haoqing Zhang; Pavel Neuzil

Rok RIV

2025

Vydáno

01.11.2023

Nakladatel

ELSEVIER SCIENCE SA

Místo

LAUSANNE

ISSN

0925-4005

Periodikum

SENSORS AND ACTUATORS B-CHEMICAL

Svazek

394

Číslo

November 2023

Stát

Švýcarská konfederace

Strany od

1

Strany do

9

Strany počet

9

URL

https://www.sciencedirect.com/science/article/pii/S0925400523010146

BibTex

@article{BUT186765,
  author="Hanliang Zhu and Xiaocheng Liu and Yingqi Wang and Antao Sun and Tomas Teply and Marie Korabecna and Haoqing Zhang and Pavel Neuzil",
  title="Smallest dual-color qPCR device",
  journal="SENSORS AND ACTUATORS B-CHEMICAL",
  year="2023",
  volume="394",
  number="November 2023",
  pages="1--9",
  doi="10.1016/j.snb.2023.134299",
  url="https://www.sciencedirect.com/science/article/pii/S0925400523010146"
}