Use of Calcium Chloride for Removal of Polysaccharides During DNA Isolation from Tropical Fruit Products

Use of Calcium Chloride for Removal of Polysaccharides During DNA Isolation from Tropical Fruit Products

Abstrakt

Food products made out of certain fruits (such as mango) may be subject to adulteration. The adulteration may be detected by various methods, including PCR-based ones. However, PCR-based methods are susceptible to inhibition if the DNA sample is contaminated with certain polysaccharides. Furthermore, polysaccharide contamination is notoriously difficult to remove. In this work we demonstrate the use of calcium chloride for successful removal of potentially inhibitory polysaccharides from samples which are known to contain them (mango and banana puree). We also address the potential inhibitory effect of calcium chloride itself and we show a way in which polysaccharide precipitation by calcium chloride may be added to a DNA isolation protocol without hampering the amplifiability of the isolated DNA.

Food products made out of certain fruits (such as mango) may be subject to adulteration. The adulteration may be detected by various methods, including PCR-based ones. However, PCR-based methods are susceptible to inhibition if the DNA sample is contaminated with certain polysaccharides. Furthermore, polysaccharide contamination is notoriously difficult to remove. In this work we demonstrate the use of calcium chloride for successful removal of potentially inhibitory polysaccharides from samples which are known to contain them (mango and banana puree). We also address the potential inhibitory effect of calcium chloride itself and we show a way in which polysaccharide precipitation by calcium chloride may be added to a DNA isolation protocol without hampering the amplifiability of the isolated DNA.

PCR, DNA isolation, polysaccharides, calcium chloride, mango

PCR, DNA isolation, polysaccharides, calcium chloride, mango

FIALOVÁ, L.; ŽYLKOVÁ, K.; MÁROVÁ, I.

23.10.2023

Institute of Analytical Chemistry of the CAS, v. v. i., Veveri 97, 602 00 Brno, Czech Republic

Brno

978-80-908154-0-7

CECE 2023 18th International Interdisciplinary Meeting on Bioanalysis

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https://www.ce-ce.org/user_uploads/CECE_2023/CECE%202023%20Proceedings.pdf

cece2023