Detail publikačního výsledku

Increasing the Image Contrast via Fast Fluorescence Photobleaching

KOLÁŘ, R.; VIČAR, T.; CHMELÍKOVÁ, L.; PROVAZNÍK, I.

Originální název

Increasing the Image Contrast via Fast Fluorescence Photobleaching

Anglický název

Increasing the Image Contrast via Fast Fluorescence Photobleaching

Druh

Stať ve sborníku v databázi WoS či Scopus

Originální abstrakt

This paper focuses on the analysis of image sequences acquired during fast photobleaching using a standard wide-field microscope. We show that the photobleaching rate estimated for each pixel is not constant for the whole field of view, but it provides a new spatially variant parametric image related to the cell structure and diffusion of fluorophores. We also provide an alternative way to estimate a pixel-wise photobleaching rate with significantly less computation time than exponential model fitting.

Anglický abstrakt

This paper focuses on the analysis of image sequences acquired during fast photobleaching using a standard wide-field microscope. We show that the photobleaching rate estimated for each pixel is not constant for the whole field of view, but it provides a new spatially variant parametric image related to the cell structure and diffusion of fluorophores. We also provide an alternative way to estimate a pixel-wise photobleaching rate with significantly less computation time than exponential model fitting.

Klíčová slova

image sequences;photobleaching;microscopy

Klíčová slova v angličtině

image sequences;photobleaching;microscopy

Autoři

KOLÁŘ, R.; VIČAR, T.; CHMELÍKOVÁ, L.; PROVAZNÍK, I.

Rok RIV

2023

Vydáno

18.11.2021

Nakladatel

Elsevier

ISBN

978-1-7281-1179-7

Kniha

2021 43nd Annual International Conference of the IEEE Engineering in Medicine & Biology Society (EMBC)

Strany od

2741

Strany do

2744

Strany počet

4

URL

https://ieeexplore.ieee.org/document/9630859

BibTex

@inproceedings{BUT173256,
  author="Radim {Kolář} and Tomáš {Vičar} and Larisa {Chmelíková} and Valentýna {Provazník}",
  title="Increasing the Image Contrast via Fast Fluorescence Photobleaching",
  booktitle="2021 43nd Annual International Conference of the IEEE Engineering in Medicine & Biology Society (EMBC)",
  year="2021",
  pages="2741--2744",
  publisher="Elsevier",
  doi="10.1109/EMBC46164.2021.9630859",
  isbn="978-1-7281-1179-7",
  url="https://ieeexplore.ieee.org/document/9630859"
}