Detail publikačního výsledku

Graphene Oxide Mimics Biological Signaling Cue to Rescue Starving Bacteria

JACKMAN, J.; YOON, B.; MOKRZECKA, N.; KOHLI, G.; VALLE-GONZALEZ, E.; ZHU, X.; PUMERA, M.; RICE, S.; CHO, N.

Originální název

Graphene Oxide Mimics Biological Signaling Cue to Rescue Starving Bacteria

Anglický název

Graphene Oxide Mimics Biological Signaling Cue to Rescue Starving Bacteria

Druh

Článek WoS

Originální abstrakt

There is extensive debate about how 2D nanomaterials such as graphene oxide (GO) affect bacteria. Various effects of GO are proposed, including bacterial growth inhibition or enhancement, killing, and no activity. Herein, we report that GO protects Staphylococcus aureus bacterial cells from death in starvation conditions with up to a 1000-fold improvement in cell viability. Transcriptomic profiling reveals that bacterial cells in starvation conditions generally shut down metabolic activity, while only cells incubated with GO increase production of specific enzymes involved in the glyoxalase detoxification pathway along with repressed autolysis. The oxygen-containing functional groups of GO resemble the molecular structure of methylglyoxal, which bacteria produce to adapt to nutrient imbalances and is detoxified by glyoxalase enzymes. The ability of GO to enable bacterial cell survival in starvation conditions and accompanying cellular responses support that bacterial cells perceive GO as a methylglyoxal-mimicking nanomaterial cue to reshuffle cellular metabolism and defenses.

Anglický abstrakt

There is extensive debate about how 2D nanomaterials such as graphene oxide (GO) affect bacteria. Various effects of GO are proposed, including bacterial growth inhibition or enhancement, killing, and no activity. Herein, we report that GO protects Staphylococcus aureus bacterial cells from death in starvation conditions with up to a 1000-fold improvement in cell viability. Transcriptomic profiling reveals that bacterial cells in starvation conditions generally shut down metabolic activity, while only cells incubated with GO increase production of specific enzymes involved in the glyoxalase detoxification pathway along with repressed autolysis. The oxygen-containing functional groups of GO resemble the molecular structure of methylglyoxal, which bacteria produce to adapt to nutrient imbalances and is detoxified by glyoxalase enzymes. The ability of GO to enable bacterial cell survival in starvation conditions and accompanying cellular responses support that bacterial cells perceive GO as a methylglyoxal-mimicking nanomaterial cue to reshuffle cellular metabolism and defenses.

Klíčová slova

bacterias; graphene oxide; nanomaterials; oxidative stress; surface functionalization

Klíčová slova v angličtině

bacterias; graphene oxide; nanomaterials; oxidative stress; surface functionalization

Autoři

JACKMAN, J.; YOON, B.; MOKRZECKA, N.; KOHLI, G.; VALLE-GONZALEZ, E.; ZHU, X.; PUMERA, M.; RICE, S.; CHO, N.

Rok RIV

2022

Vydáno

01.06.2021

Nakladatel

WILEY-V C H VERLAG GMBH

Místo

WEINHEIM

ISSN

1616-301X

Periodikum

ADVANCED FUNCTIONAL MATERIALS

Svazek

31

Číslo

25

Stát

Spolková republika Německo

Strany od

2102328-1

Strany do

2102328-9

Strany počet

7

URL