Detail publikačního výsledku

Nanowatt simple microcalorimetry for dynamically monitoring the defense mechanism of Paramecium caudatum

FENG, J.; ZHU, H.; LUKEŠ, J.; KORABEČNÁ, M.; FOHLEROVÁ, Z.; MEI, T.; CHANG, H.; NEUŽIL, P.

Originální název

Nanowatt simple microcalorimetry for dynamically monitoring the defense mechanism of Paramecium caudatum

Anglický název

Nanowatt simple microcalorimetry for dynamically monitoring the defense mechanism of Paramecium caudatum

Druh

Článek WoS

Originální abstrakt

Microcalorimetry has been widely used in measuring cellular metabolic heat to study bioprocesses, such as metabolism. However, it is still limited by insufficient sensitivity and system complexity, especially for the direct measurements of individual cells. Here, we present a droplet-based simple differential microcalorimetric system for determining the real-time energy balance of the ciliate protist, Paramecium caudatum. We utilized the platform to dynamically monitor its defensive behavior and measured the temperature change. Then we used heat balance equation and calculated corresponding dissipated power and energy with an ultimate resolution of kl14 nW. The results showed that the defensive behavior by the ejection of the trichocysts & rsquo; content consumed energy of kl0.75 mJ per cell, and the dissipated power was kl303.8 & micro;W. This differential microcalorimetric platform can be used to study the metabolic heat of protist metabolism as well as other individual cells, helping us to understand this widespread, yet little-known, biological phenomenon from a new perspective. (c) 2021 Elsevier B.V. All rights reserved.

Anglický abstrakt

Microcalorimetry has been widely used in measuring cellular metabolic heat to study bioprocesses, such as metabolism. However, it is still limited by insufficient sensitivity and system complexity, especially for the direct measurements of individual cells. Here, we present a droplet-based simple differential microcalorimetric system for determining the real-time energy balance of the ciliate protist, Paramecium caudatum. We utilized the platform to dynamically monitor its defensive behavior and measured the temperature change. Then we used heat balance equation and calculated corresponding dissipated power and energy with an ultimate resolution of kl14 nW. The results showed that the defensive behavior by the ejection of the trichocysts & rsquo; content consumed energy of kl0.75 mJ per cell, and the dissipated power was kl303.8 & micro;W. This differential microcalorimetric platform can be used to study the metabolic heat of protist metabolism as well as other individual cells, helping us to understand this widespread, yet little-known, biological phenomenon from a new perspective. (c) 2021 Elsevier B.V. All rights reserved.

Klíčová slova

Microcalorimetry; Energy balance monitoring; Defense mechanism; Metabolic heat; Unicellular organism

Klíčová slova v angličtině

Microcalorimetry; Energy balance monitoring; Defense mechanism; Metabolic heat; Unicellular organism

Autoři

FENG, J.; ZHU, H.; LUKEŠ, J.; KORABEČNÁ, M.; FOHLEROVÁ, Z.; MEI, T.; CHANG, H.; NEUŽIL, P.

Rok RIV

2021

Vydáno

22.04.2021

Nakladatel

ELSEVIER SCIENCE SA

Místo

LAUSANNE

ISSN

0924-4247

Periodikum

SENSORS AND ACTUATORS A-PHYSICAL

Svazek

323

Číslo

1

Stát

Švýcarská konfederace

Strany od

1

Strany do

9

Strany počet

9

URL

http://apps.webofknowledge.com/full_record.do?product=WOS&search_mode=GeneralSearch&qid=3&SID=E44ZFBnYg876vQecPbL&page=1&doc=1

BibTex

@article{BUT171297,
  author="Jianguo {Feng} and Hanliang {Zhu} and Julius {Lukeš} and Marie {Korabečná} and Zdenka {Fohlerová} and Ting {Mei} and Honglong {Chang} and Pavel {Neužil}",
  title="Nanowatt simple microcalorimetry for dynamically monitoring the defense mechanism of Paramecium caudatum",
  journal="SENSORS AND ACTUATORS A-PHYSICAL",
  year="2021",
  volume="323",
  number="1",
  pages="1--9",
  doi="10.1016/j.sna.2021.112643",
  issn="0924-4247",
  url="http://apps.webofknowledge.com/full_record.do?product=WOS&search_mode=GeneralSearch&qid=3&SID=E44ZFBnYg876vQecPbL&page=1&doc=1"
}