Detail publikačního výsledku

Surface-enhanced Raman Spectroscopy in Microfluidic Chips for Directed Evolution of Enzymes and Environmental Monitoring

Pilat, Zdenek; Jezek, Jan; Kizovsky, Martin; Klementova, Tereza; Kratky, Stanislav; Sobota, Jaroslav; Samek, Ota; Zemanek, Pavel; Buryska, Tomas; Damborsky, Jiri; Prokop, Zbynek

Originální název

Surface-enhanced Raman Spectroscopy in Microfluidic Chips for Directed Evolution of Enzymes and Environmental Monitoring

Anglický název

Surface-enhanced Raman Spectroscopy in Microfluidic Chips for Directed Evolution of Enzymes and Environmental Monitoring

Druh

Článek WoS

Originální abstrakt

Optofluidics, a research discipline combining optics with microfluidics, currently aspires to revolutionize the analysis of biological and chemical samples e.g. for medicine, pharmacology, or molecular biology. In order to detect low concentrations of analytes in water, we developed an optofluidic device containing a nanostructured substrate for surface enhanced Raman spectroscopy (SERS). The geometry of the gold surface allows localized plasmon oscillations to give rise to the SERS effect, in which the Raman spectral lines are intensified by the interaction of the plasmonic field with the electrons in the molecular bonds. The SERS substrate was enclosed in a microfluidic system, which allowed transport and precise mixing of the analyzed fluids, while preventing contamination or abrasion of the highly sensitive substrate. To illustrate its practical use, we employed the device for quantitative detection of persistent environmental pollutant 1,2,3-trichloropropane in water in millimolar and even submillimolar concentrations. The developed sensor allows fast and simple quantification of halogenated compounds and it will contribute towards the environmental monitoring and enzymology experiments with engineered haloalkane dehalogenase enzymes.

Anglický abstrakt

Optofluidics, a research discipline combining optics with microfluidics, currently aspires to revolutionize the analysis of biological and chemical samples e.g. for medicine, pharmacology, or molecular biology. In order to detect low concentrations of analytes in water, we developed an optofluidic device containing a nanostructured substrate for surface enhanced Raman spectroscopy (SERS). The geometry of the gold surface allows localized plasmon oscillations to give rise to the SERS effect, in which the Raman spectral lines are intensified by the interaction of the plasmonic field with the electrons in the molecular bonds. The SERS substrate was enclosed in a microfluidic system, which allowed transport and precise mixing of the analyzed fluids, while preventing contamination or abrasion of the highly sensitive substrate. To illustrate its practical use, we employed the device for quantitative detection of persistent environmental pollutant 1,2,3-trichloropropane in water in millimolar and even submillimolar concentrations. The developed sensor allows fast and simple quantification of halogenated compounds and it will contribute towards the environmental monitoring and enzymology experiments with engineered haloalkane dehalogenase enzymes.

Klíčová slova

Substrates; Springs; Raman scattering; Photonics; Electromagnetics; Measurement by laser beam; Gold

Klíčová slova v angličtině

Substrates; Springs; Raman scattering; Photonics; Electromagnetics; Measurement by laser beam; Gold

Autoři

Pilat, Zdenek; Jezek, Jan; Kizovsky, Martin; Klementova, Tereza; Kratky, Stanislav; Sobota, Jaroslav; Samek, Ota; Zemanek, Pavel; Buryska, Tomas; Damborsky, Jiri; Prokop, Zbynek

Rok RIV

2021

Vydáno

02.03.2020

Nakladatel

IEEE

Místo

NEW YORK

ISBN

1559-9450

Kniha

2019 PhotonIcs & Electromagnetics Research Symposium - Spring (PIERS-Spring)

ISSN

1559-9450

Periodikum

Progress In Electromagnetics

Svazek

2019

Číslo

1

Stát

Spojené státy americké

Strany od

1301

Strany do

1309

Strany počet

9

URL

https://ieeexplore-ieee-org.ezproxy.lib.vutbr.cz/document/9017521

BibTex

@article{BUT170289,
  author="Pilat, Zdenek and Jezek, Jan and Kizovsky, Martin and Klementova, Tereza and Kratky, Stanislav and Sobota, Jaroslav and Samek, Ota and Zemanek, Pavel and Buryska, Tomas and Damborsky, Jiri and Prokop, Zbynek",
  title="Surface-enhanced Raman Spectroscopy in Microfluidic Chips for Directed Evolution of Enzymes and Environmental Monitoring",
  journal="Progress In Electromagnetics",
  year="2020",
  volume="2019",
  number="1",
  pages="1301--1309",
  doi="10.1109/PIERS-Spring46901.2019.9017521",
  issn="1559-9450",
  url="https://ieeexplore-ieee-org.ezproxy.lib.vutbr.cz/document/9017521"
}