Detail publikačního výsledku

PCR past, present and future

ZHU, H.; ZHANG, H.; XU, Y.; LAŠŠÁKOVÁ, S.; KORABEČNÁ, M.; NEUŽIL, P.

Originální název

PCR past, present and future

Anglický název

PCR past, present and future

Druh

Článek WoS

Originální abstrakt

PCR has become one of the most valuable techniques currently used in bioscience, diagnostics and forensic science. Here we review the history of PCR development and the technologies that have evolved from the original PCR method. Currently, there are two main areas of PCR utilization in bioscience: high-throughput PCR systems and microfluidics-based PCR devices for point-of-care (POC) applications. We also discuss the commercialization of these techniques and conclude with a look into their modifications and use in innovative areas of biomedicine. For example, real-time reverse transcription PCR is the gold standard for SARS-CoV-2 diagnoses. It could also be used for POC applications, being a key component of the sample-to-answer system.

Anglický abstrakt

PCR has become one of the most valuable techniques currently used in bioscience, diagnostics and forensic science. Here we review the history of PCR development and the technologies that have evolved from the original PCR method. Currently, there are two main areas of PCR utilization in bioscience: high-throughput PCR systems and microfluidics-based PCR devices for point-of-care (POC) applications. We also discuss the commercialization of these techniques and conclude with a look into their modifications and use in innovative areas of biomedicine. For example, real-time reverse transcription PCR is the gold standard for SARS-CoV-2 diagnoses. It could also be used for POC applications, being a key component of the sample-to-answer system.

Klíčová slova

COVID-19; digital PCR; microfluidics; PCR; point-of-care diagnostics; portable systems; reverse transcription PCR; RNA virus diagnoses

Klíčová slova v angličtině

COVID-19; digital PCR; microfluidics; PCR; point-of-care diagnostics; portable systems; reverse transcription PCR; RNA virus diagnoses

Autoři

ZHU, H.; ZHANG, H.; XU, Y.; LAŠŠÁKOVÁ, S.; KORABEČNÁ, M.; NEUŽIL, P.

Rok RIV

2021

Vydáno

20.08.2020

Nakladatel

FUTURE SCI LTD

Místo

LONDON

ISSN

0736-6205

Periodikum

BIOTECHNIQUES

Svazek

69

Číslo

4

Stát

Spojené státy americké

Strany od

1

Strany do

10

Strany počet

10

URL

https://www.future-science.com/doi/full/10.2144/btn-2020-0057

BibTex

@article{BUT165710,
  author="ZHU, H. and ZHANG, H. and XU, Y. and LAŠŠÁKOVÁ, S. and KORABEČNÁ, M. and NEUŽIL, P.",
  title="PCR past, present and future",
  journal="BIOTECHNIQUES",
  year="2020",
  volume="69",
  number="4",
  pages="1--10",
  doi="10.2144/btn-2020-0057",
  issn="0736-6205",
  url="https://www.future-science.com/doi/full/10.2144/btn-2020-0057"
}