Detail publikačního výsledku

Optical topography of rough surfaces using vortex localization of fluorescent markers

SCHOVÁNEK, P.; BOUCHAL, P.; BOUCHAL, Z.

Originální název

Optical topography of rough surfaces using vortex localization of fluorescent markers

Anglický název

Optical topography of rough surfaces using vortex localization of fluorescent markers

Druh

Článek WoS

Originální abstrakt

Measuring rough surfaces is challenging because the proven topographic methods are impaired by the adverse effects of diffuse light. In our method, the measured surface is marked by fluorescent nanobeads allowing a complete suppression of diffuse light by bandpass filtering. Light emitted by each fluorescent bead is shaped to a double-helix point spread function used for three-dimensional bead localization on the surface. This non-interferometric measurement of rough surface topography is implemented in a vibration resistant setup. The comparison of our method with vertical scanning interferometry shows that a commercial profiler is surpassed when ground glass surfaces with steep slopes are measured. (C) 2020 Optical Society of America

Anglický abstrakt

Measuring rough surfaces is challenging because the proven topographic methods are impaired by the adverse effects of diffuse light. In our method, the measured surface is marked by fluorescent nanobeads allowing a complete suppression of diffuse light by bandpass filtering. Light emitted by each fluorescent bead is shaped to a double-helix point spread function used for three-dimensional bead localization on the surface. This non-interferometric measurement of rough surface topography is implemented in a vibration resistant setup. The comparison of our method with vertical scanning interferometry shows that a commercial profiler is surpassed when ground glass surfaces with steep slopes are measured. (C) 2020 Optical Society of America

Klíčová slova

optical vortices, nondiffracting beams, optical topography, localization microscopy

Klíčová slova v angličtině

optical vortices, nondiffracting beams, optical topography, localization microscopy

Autoři

SCHOVÁNEK, P.; BOUCHAL, P.; BOUCHAL, Z.

Rok RIV

2021

Vydáno

15.08.2020

Nakladatel

OPTICAL SOC AMER

Místo

WASHINGTON

ISSN

1539-4794

Periodikum

OPTICS LETTERS

Svazek

45

Číslo

16

Stát

Spojené státy americké

Strany od

4468

Strany do

4471

Strany počet

4

URL

https://www.osapublishing.org/ol/abstract.cfm?uri=ol-45-16-4468

Plný text v Digitální knihovně

http://hdl.handle.net/

BibTex

@article{BUT164672,
  author="Petr {Schovánek} and Petr {Bouchal} and Zdeněk {Bouchal}",
  title="Optical topography of rough surfaces using vortex localization of fluorescent markers",
  journal="OPTICS LETTERS",
  year="2020",
  volume="45",
  number="16",
  pages="4468--4471",
  doi="10.1364/OL.392072",
  issn="0146-9592",
  url="https://www.osapublishing.org/ol/abstract.cfm?uri=ol-45-16-4468"
}