Detail publikačního výsledku

Isolation of branched chain aminoacids (Valine, Leucine and Arginine) using maghemite particles

CERNEI, N.; KOPEL, P.; HORKÝ, P.; HYNEK, D.; ZÍTKA, O.; ADAM, V.

Originální název

Isolation of branched chain aminoacids (Valine, Leucine and Arginine) using maghemite particles

Anglický název

Isolation of branched chain aminoacids (Valine, Leucine and Arginine) using maghemite particles

Druh

Stať ve sborníku v databázi WoS či Scopus

Originální abstrakt

In this paper, we report the optimal conditions for the isolation and preconcentration of branched chain amino acids (BCAAs) - leucine, valine and arginine from different types of matrix, such as tissue, plasma, blood, bacteria or cells. Herein, we focused on the synthesis of paramagnetic particles able to isolate and immobilize amino acids and thus preconcentrate it for subsequent analysis on ion-exchange liquid chromatography with VIS detector. We modified nanomaghemite (γ-Fe2O3) by multi walled carbon nanotubes (MWCNT) and hyaluronic acid as the functional carriers providing excellent affinity properties. Our paramagnetic particles have potential for better isolation of BCAAs mainly from plasma or bacteria and in the future they can also be applied as a platform of delivery system.

Anglický abstrakt

In this paper, we report the optimal conditions for the isolation and preconcentration of branched chain amino acids (BCAAs) - leucine, valine and arginine from different types of matrix, such as tissue, plasma, blood, bacteria or cells. Herein, we focused on the synthesis of paramagnetic particles able to isolate and immobilize amino acids and thus preconcentrate it for subsequent analysis on ion-exchange liquid chromatography with VIS detector. We modified nanomaghemite (γ-Fe2O3) by multi walled carbon nanotubes (MWCNT) and hyaluronic acid as the functional carriers providing excellent affinity properties. Our paramagnetic particles have potential for better isolation of BCAAs mainly from plasma or bacteria and in the future they can also be applied as a platform of delivery system.

Klíčová slova

Ion Exchange Chromatography; Maghemite; MWCNT; BCAAs; valine; leucine; arginine

Klíčová slova v angličtině

Ion Exchange Chromatography; Maghemite; MWCNT; BCAAs; valine; leucine; arginine

Autoři

CERNEI, N.; KOPEL, P.; HORKÝ, P.; HYNEK, D.; ZÍTKA, O.; ADAM, V.

Rok RIV

2021

Vydáno

10.06.2020

Nakladatel

Tanger

ISBN

978-80-87294-95-6

Kniha

NANOCON 2019 CONFERENCE PROCEEDINGS

Edice

1.

Strany od

374

Strany do

378

Strany počet

5

URL

https://doi.org/10.37904/nanocon.2019.8497

Plný text v Digitální knihovně

http://hdl.handle.net/11012/196672

BibTex

@inproceedings{BUT164259,
  author="Natalia Vladimirovna {Cernei} and Pavel {Kopel} and Pavel {Horký} and David {Hynek} and Ondřej {Zítka} and Vojtěch {Adam}",
  title="Isolation of branched chain aminoacids (Valine, Leucine and Arginine) using maghemite particles",
  booktitle="NANOCON 2019 CONFERENCE PROCEEDINGS",
  year="2020",
  series="1.",
  pages="374--378",
  publisher="Tanger",
  doi="10.37904/nanocon.2019.8497",
  isbn="978-80-87294-95-6",
  url="https://doi.org/10.37904/nanocon.2019.8497"
}

Dokumenty

nanocon2019_cernei_nanocon