Detail publikačního výsledku

Magnetic molecularly imprinted polymers used for selective isolation and detection of Staphylococcus aureus

BEZDĚKOVÁ, J.; ZEMÁNKOVÁ, K.; HUTAŘOVÁ, J.; KOČIOVÁ, S.; ŠMERKOVÁ, K.; ADAM, V.; VACULOVIČOVÁ, M.

Originální název

Magnetic molecularly imprinted polymers used for selective isolation and detection of Staphylococcus aureus

Anglický název

Magnetic molecularly imprinted polymers used for selective isolation and detection of Staphylococcus aureus

Druh

Článek WoS

Originální abstrakt

In this work, a novel method was developed, for isolation of S. aureus from complex (food) samples using molecular imprinting. Dopamine was used as a functional monomer and fluorescence microscopy was used for detection. Conditions for preparation of molecularly imprinted polymers (MIPs), adsorption performance, adsorption kinetic, and selectivity of the polymeric layers were investigated. The various procedures were combined in a single extraction process, with the imprinted layer on the surface of the magnetic particles (magnetic MIPs). Subsequently, MIPs were used for extraction of S. aureus from milk and rice. Moreover, raw milk from cows with mastitis was tested successfully. Using this novel MIP-based method, it was possible to detect bacteria in milk at 1 x 10(3) CFU.ml(-1), which corresponds to the limit set in European Union legislation for microbial control of food.

Anglický abstrakt

In this work, a novel method was developed, for isolation of S. aureus from complex (food) samples using molecular imprinting. Dopamine was used as a functional monomer and fluorescence microscopy was used for detection. Conditions for preparation of molecularly imprinted polymers (MIPs), adsorption performance, adsorption kinetic, and selectivity of the polymeric layers were investigated. The various procedures were combined in a single extraction process, with the imprinted layer on the surface of the magnetic particles (magnetic MIPs). Subsequently, MIPs were used for extraction of S. aureus from milk and rice. Moreover, raw milk from cows with mastitis was tested successfully. Using this novel MIP-based method, it was possible to detect bacteria in milk at 1 x 10(3) CFU.ml(-1), which corresponds to the limit set in European Union legislation for microbial control of food.

Klíčová slova

Molecularly imprinted polymers; Magnetic particles; Dopamine; Bacteria; Fluorescence microscopy

Klíčová slova v angličtině

Molecularly imprinted polymers; Magnetic particles; Dopamine; Bacteria; Fluorescence microscopy

Autoři

BEZDĚKOVÁ, J.; ZEMÁNKOVÁ, K.; HUTAŘOVÁ, J.; KOČIOVÁ, S.; ŠMERKOVÁ, K.; ADAM, V.; VACULOVIČOVÁ, M.

Rok RIV

2021

Vydáno

15.08.2020

ISSN

0308-8146

Periodikum

Food Chemistry

Svazek

321

Číslo

126673

Stát

Spojené království Velké Británie a Severního Irska

Strany od

1

Strany do

8

Strany počet

8

URL

https://www.sciencedirect.com/science/article/abs/pii/S0308814620305355

BibTex

@article{BUT164091,
  author="Jaroslava {Bezděková} and Kristýna {Zemánková} and Jitka {Hutařová} and Silvia {Kočiová} and Kristýna {Šmerková} and Vojtěch {Adam} and Markéta {Vaculovičová}",
  title="Magnetic molecularly imprinted polymers used for selective isolation and detection of Staphylococcus aureus",
  journal="Food Chemistry",
  year="2020",
  volume="321",
  number="126673",
  pages="1--8",
  doi="10.1016/j.foodchem.2020.126673",
  issn="0308-8146",
  url="https://www.sciencedirect.com/science/article/abs/pii/S0308814620305355"
}

Dokumenty

Magnetic molecularly imprinted polymers used for selective isolation and detection of Staphylococcus aureus