Detail publikačního výsledku

Structure investigation of hydrogels using a cryo-SEM

ADÁMKOVÁ, K.; HRUBANOVÁ, K.; SAMEK, O.; TRUDIČOVÁ, M.; SEDLÁČEK, P.; KRZYŽÁNEK, V.

Originální název

Structure investigation of hydrogels using a cryo-SEM

Anglický název

Structure investigation of hydrogels using a cryo-SEM

Druh

Stať ve sborníku v databázi WoS či Scopus

Originální abstrakt

The aim of this study is to examine and describe the ultrastructure of a selected hydrogel, namely hyaluronic acid and agarose. Several types of low temperature sample preparation protocols were compared. Especially, the effect of various freeze-etching times was investigated. In addition, besides the ultrastructural characterization by cryo-SEM the chemical analysis of selected samples was performed by Raman spectroscopy (RS). RS is an optical method enabling chemical analysis of a microscopic sample by laser beam. Fast and reliable monitoring and identification of chemical additives specific for selected hydrogels is performed by a library of spectra in the so-called fingerprint area. Experimental results can contribute to the widening of knowledge about the influence of hydrogel composition on its structure. The comparison of selected techniques can be recognized as suitable for the structural and chemical analysis applicable to other hydrogel systems as well.

Anglický abstrakt

The aim of this study is to examine and describe the ultrastructure of a selected hydrogel, namely hyaluronic acid and agarose. Several types of low temperature sample preparation protocols were compared. Especially, the effect of various freeze-etching times was investigated. In addition, besides the ultrastructural characterization by cryo-SEM the chemical analysis of selected samples was performed by Raman spectroscopy (RS). RS is an optical method enabling chemical analysis of a microscopic sample by laser beam. Fast and reliable monitoring and identification of chemical additives specific for selected hydrogels is performed by a library of spectra in the so-called fingerprint area. Experimental results can contribute to the widening of knowledge about the influence of hydrogel composition on its structure. The comparison of selected techniques can be recognized as suitable for the structural and chemical analysis applicable to other hydrogel systems as well.

Klíčová slova

hydrogel, cryo-SEM, agarose, hyaluronic acid

Klíčová slova v angličtině

hydrogel, cryo-SEM, agarose, hyaluronic acid

Autoři

ADÁMKOVÁ, K.; HRUBANOVÁ, K.; SAMEK, O.; TRUDIČOVÁ, M.; SEDLÁČEK, P.; KRZYŽÁNEK, V.

Rok RIV

2020

Vydáno

01.01.2018

Nakladatel

ACAD SCIENCES, CZECH REPUBLIC, INST SCIENTIFIC INSTRUMENTS ASCR, V V I, KRALOVOPOLSKA 147, BRNO, 612 64, CZECH REPUBLIC

Místo

Brno, CZECH REPUBLIC

ISBN

978-80-87441-23-7

Kniha

16th International Seminar of the Institute-of-Scientific-Instruments-The-Czech-Academy-of-Sciences

Edice

1

Strany od

26

Strany do

28

Strany počet

3

BibTex

@inproceedings{BUT163790,
  author="ADÁMKOVÁ, K. and HRUBANOVÁ, K. and SAMEK, O. and TRUDIČOVÁ, M. and SEDLÁČEK, P. and KRZYŽÁNEK, V.",
  title="Structure investigation of hydrogels using a cryo-SEM",
  booktitle="16th International Seminar of the Institute-of-Scientific-Instruments-The-Czech-Academy-of-Sciences",
  year="2018",
  series="1",
  number="1",
  pages="26--28",
  publisher="ACAD SCIENCES, CZECH REPUBLIC, INST SCIENTIFIC INSTRUMENTS ASCR, V V I, KRALOVOPOLSKA 147, BRNO, 612 64, CZECH REPUBLIC",
  address="Brno, CZECH REPUBLIC",
  isbn="978-80-87441-23-7"
}