Detail publikačního výsledku

Cell segmentation methods for label-free contrast microscopy: review and comprehensive comparison

VIČAR, T.; BALVAN, J.; JAROŠ, J.; JUG, F.; KOLÁŘ, R.; MASAŘÍK, M.; GUMULEC, J.

Originální název

Cell segmentation methods for label-free contrast microscopy: review and comprehensive comparison

Anglický název

Cell segmentation methods for label-free contrast microscopy: review and comprehensive comparison

Druh

Článek WoS

Originální abstrakt

Because of its non-destructive nature, label-free imaging is an important strategy for studying biological processes. However, routine microscopic techniques like phase contrast or DIC suffer from shadow-cast artifacts making automatic segmentation challenging. The aim of this study was to compare the segmentation efficacy of published steps of segmentation work-flow (image reconstruction, foreground segmentation, cell detection (seed-point extraction) and cell (instance) segmentation) on a dataset of the same cells from multiple contrast microscopic modalities.

Anglický abstrakt

Because of its non-destructive nature, label-free imaging is an important strategy for studying biological processes. However, routine microscopic techniques like phase contrast or DIC suffer from shadow-cast artifacts making automatic segmentation challenging. The aim of this study was to compare the segmentation efficacy of published steps of segmentation work-flow (image reconstruction, foreground segmentation, cell detection (seed-point extraction) and cell (instance) segmentation) on a dataset of the same cells from multiple contrast microscopic modalities.

Klíčová slova

Microscopy, Cell segmentation, Image reconstruction, Methods comparison, Differential contrast image, Quantitative phase imaging, Laplacian of Gaussians

Klíčová slova v angličtině

Microscopy, Cell segmentation, Image reconstruction, Methods comparison, Differential contrast image, Quantitative phase imaging, Laplacian of Gaussians

Autoři

VIČAR, T.; BALVAN, J.; JAROŠ, J.; JUG, F.; KOLÁŘ, R.; MASAŘÍK, M.; GUMULEC, J.

Rok RIV

2020

Vydáno

28.07.2019

Nakladatel

BMC

Místo

London, England

ISSN

1471-2105

Periodikum

BMC BIOINFORMATICS

Svazek

20

Číslo

1

Stát

Spojené království Velké Británie a Severního Irska

Strany od

1

Strany do

25

Strany počet

25

URL

https://bmcbioinformatics.biomedcentral.com/articles/10.1186/s12859-019-2880-8

Plný text v Digitální knihovně

http://hdl.handle.net/11012/195836

BibTex

@article{BUT158175,
  author="Tomáš {Vičar} and Jan {Balvan} and Josef {Jaroš} and Florian {Jug} and Radim {Kolář} and Michal {Masařík} and Jaromír {Gumulec}",
  title="Cell segmentation methods for label-free contrast microscopy: review and comprehensive comparison",
  journal="BMC BIOINFORMATICS",
  year="2019",
  volume="20",
  number="1",
  pages="1--25",
  doi="10.1186/s12859-019-2880-8",
  issn="1471-2105",
  url="https://bmcbioinformatics.biomedcentral.com/articles/10.1186/s12859-019-2880-8"
}

Dokumenty

s12859-019-2880-8