Detail publikačního výsledku

Attenuation of p53-alpha isoform binding to DNA by inverted repeat abolishment in p53 target

PORUBIAKOVÁ, O.; BOHÁLOVÁ, N.; BRÁZDA, V.; INGA, A.

Originální název

Attenuation of p53-alpha isoform binding to DNA by inverted repeat abolishment in p53 target

Anglický název

Attenuation of p53-alpha isoform binding to DNA by inverted repeat abolishment in p53 target

Druh

Abstrakt

Originální abstrakt

Protein p53 is one of the most studied tumour suppressor proteins, plays important roles in regulating basic biological processes including cell cycle, apoptosis, senescence and metabolism. P53 protein function is realized by binding to specific DNA response elements and transactivation of target genes. We presented results of p53alpha isoform DNA interaction studied by yeast isogenic system for in vivo transactivation studies in chromosomal context. This variable system is applicable for analyses of influence of secondary DNA structures to protein-DNA binding in vivo.

Anglický abstrakt

Protein p53 is one of the most studied tumour suppressor proteins, plays important roles in regulating basic biological processes including cell cycle, apoptosis, senescence and metabolism. P53 protein function is realized by binding to specific DNA response elements and transactivation of target genes. We presented results of p53alpha isoform DNA interaction studied by yeast isogenic system for in vivo transactivation studies in chromosomal context. This variable system is applicable for analyses of influence of secondary DNA structures to protein-DNA binding in vivo.

Klíčová slova

p53-alpha isoform, transactivation, p53 target sites, inverted repeats

Klíčová slova v angličtině

p53-alpha isoform, transactivation, p53 target sites, inverted repeats

Autoři

PORUBIAKOVÁ, O.; BOHÁLOVÁ, N.; BRÁZDA, V.; INGA, A.

Vydáno

15.05.2019

BibTex

@misc{BUT157773,
  author="Otília {Porubiaková} and Natália {Bohálová} and Václav {Brázda} and Alberto {Inga}",
  title="Attenuation of p53-alpha isoform binding to DNA by inverted repeat abolishment in p53 target",
  year="2019",
  note="Abstract"
}