Detail publikačního výsledku

The 3D imaging of mesenchymal stem cells on porous scaffolds using high-contrasted x-ray computed nanotomography

VOJTOVÁ, L.; ZIKMUND, T.; PAVLIŇÁKOVÁ, V.; ŠALPLACHTA, J.; KALASOVÁ, D.; PROSECKÁ, E.; BRTNÍKOVÁ, J.; ŽÍDEK, J.; PAVLIŇÁK, D.; KAISER, J.

Originální název

The 3D imaging of mesenchymal stem cells on porous scaffolds using high-contrasted x-ray computed nanotomography

Anglický název

The 3D imaging of mesenchymal stem cells on porous scaffolds using high-contrasted x-ray computed nanotomography

Druh

Článek WoS

Originální abstrakt

This study presents an X-ray computed nanotomography (nano-CT) based, high-resolution imaging technique. Thanks to a voxel resolution of 540 nm, this novel technique is suitable for observing the 3D morphology of soft biopolymeric scaffolds seeded with stem cells. A sample of highly porous collagen scaffold seeded with contrasted mesenchymal stem cells (MSC) was investigated by using lab-based nano-CT. The whole volume of the sample was analysed without its destruction. To evaluate the potential of nano-CT, a comparison measurement was done using a standard microscopy technique. Scanning electron microscopy (SEM) combined with energy dispersive X-ray analysis (EDX) established an extension and local accumulation of the contrasting agent – heavy metallic osmium tetroxide. The presented imaging technique is novel as it will help to understand better the behaviour of cells while interacting with three-dimensional biomaterials. This is crucial for both experimental and clinical tissue engineering applications in order to limit the risk of uncontrolled cell growth, and potentially tumour formation.

Anglický abstrakt

This study presents an X-ray computed nanotomography (nano-CT) based, high-resolution imaging technique. Thanks to a voxel resolution of 540 nm, this novel technique is suitable for observing the 3D morphology of soft biopolymeric scaffolds seeded with stem cells. A sample of highly porous collagen scaffold seeded with contrasted mesenchymal stem cells (MSC) was investigated by using lab-based nano-CT. The whole volume of the sample was analysed without its destruction. To evaluate the potential of nano-CT, a comparison measurement was done using a standard microscopy technique. Scanning electron microscopy (SEM) combined with energy dispersive X-ray analysis (EDX) established an extension and local accumulation of the contrasting agent – heavy metallic osmium tetroxide. The presented imaging technique is novel as it will help to understand better the behaviour of cells while interacting with three-dimensional biomaterials. This is crucial for both experimental and clinical tissue engineering applications in order to limit the risk of uncontrolled cell growth, and potentially tumour formation.

Klíčová slova

Biopolymeric scaffold, mesenchymal stem cells, SEM/EDX, tissue engineering, X-ray computed nanotomography

Klíčová slova v angličtině

Biopolymeric scaffold, mesenchymal stem cells, SEM/EDX, tissue engineering, X-ray computed nanotomography

Autoři

VOJTOVÁ, L.; ZIKMUND, T.; PAVLIŇÁKOVÁ, V.; ŠALPLACHTA, J.; KALASOVÁ, D.; PROSECKÁ, E.; BRTNÍKOVÁ, J.; ŽÍDEK, J.; PAVLIŇÁK, D.; KAISER, J.

Rok RIV

2019

Vydáno

01.03.2019

Nakladatel

WILEY

Místo

HOBOKEN, USA

ISSN

1365-2818

Periodikum

JOURNAL OF MICROSCOPY

Svazek

273

Číslo

3

Stát

Spojené království Velké Británie a Severního Irska

Strany od

169

Strany do

177

Strany počet

9

URL

https://onlinelibrary.wiley.com/doi/full/10.1111/jmi.12771

BibTex

@article{BUT151641,
  author="VOJTOVÁ, L. and ZIKMUND, T. and PAVLIŇÁKOVÁ, V. and ŠALPLACHTA, J. and KALASOVÁ, D. and PROSECKÁ, E. and BRTNÍKOVÁ, J. and ŽÍDEK, J. and PAVLIŇÁK, D. and KAISER, J.",
  title="The 3D imaging of mesenchymal stem cells on porous scaffolds using high-contrasted x-ray computed nanotomography",
  journal="JOURNAL OF MICROSCOPY",
  year="2019",
  volume="273",
  number="3",
  pages="169--177",
  doi="10.1111/jmi.12771",
  issn="0022-2720",
  url="https://onlinelibrary.wiley.com/doi/full/10.1111/jmi.12771"
}