Detail publikačního výsledku

ISOLATION AND DETERMINATION OF LACTOFERRINE FROM HUMAN SALIVA USING PARAMAGNETIC PARTICLES

SKALIČKOVÁ, S.; ZÍTKA, O.; KŘÍŽKOVÁ, S.; VLKOVÁ, M.; KIZEK, R.

Originální název

ISOLATION AND DETERMINATION OF LACTOFERRINE FROM HUMAN SALIVA USING PARAMAGNETIC PARTICLES

Anglický název

ISOLATION AND DETERMINATION OF LACTOFERRINE FROM HUMAN SALIVA USING PARAMAGNETIC PARTICLES

Druh

Stať ve sborníku v databázi WoS či Scopus

Originální abstrakt

Saliva is a watery substance produced in the mouths of human and most animals. The important compound of saliva is lactoferrin. It is involved in binding and transport of metal ions such as Fe2+, Zn2+, Mn2+ and Cu2+. Because of these functions it has an antibacterial, anticancerogenic and anti-inflammatory activity. The aim of this study was to design a method based on isolation of lactoferrin from human saliva using paramagnetic particles to prepare bead-based complex with lactoferrin. This complex was subsequently determined by SFIA. Our results were correlated with ELISA using spectrophotometric detection. The limit of detection for lactoferin was 0.4 mu g.ml(-1). This method has the potential as a biosensor analysis.

Anglický abstrakt

Saliva is a watery substance produced in the mouths of human and most animals. The important compound of saliva is lactoferrin. It is involved in binding and transport of metal ions such as Fe2+, Zn2+, Mn2+ and Cu2+. Because of these functions it has an antibacterial, anticancerogenic and anti-inflammatory activity. The aim of this study was to design a method based on isolation of lactoferrin from human saliva using paramagnetic particles to prepare bead-based complex with lactoferrin. This complex was subsequently determined by SFIA. Our results were correlated with ELISA using spectrophotometric detection. The limit of detection for lactoferin was 0.4 mu g.ml(-1). This method has the potential as a biosensor analysis.

Klíčová slova

lactoferrin; ELISA; SFIA; biosensor

Klíčová slova v angličtině

lactoferrin; ELISA; SFIA; biosensor

Autoři

SKALIČKOVÁ, S.; ZÍTKA, O.; KŘÍŽKOVÁ, S.; VLKOVÁ, M.; KIZEK, R.

Rok RIV

2018

Vydáno

22.11.2012

ISBN

978-80-7375-836-3

Kniha

MENDELNET 2012

Strany od

1250

Strany do

1257

Strany počet

8

BibTex

@inproceedings{BUT145755,
  author="Sylvie {Skaličková} and Ondřej {Zítka} and Soňa {Křížková} and Marcela {Vlková} and René {Kizek}",
  title="ISOLATION AND DETERMINATION OF LACTOFERRINE FROM HUMAN SALIVA USING PARAMAGNETIC PARTICLES",
  booktitle="MENDELNET 2012",
  year="2012",
  pages="1250--1257",
  isbn="978-80-7375-836-3"
}