Detail publikačního výsledku

Studying of quantum dot luminescence quenching effect caused by covalent conjugation with protein

PEJOVIČ SIMEUNOVIČ, J.; PEKÁRKOVÁ, J.; ŽÁK, J.; CHAMRADOVÁ, I.; HUBÁLEK, J.

Originální název

Studying of quantum dot luminescence quenching effect caused by covalent conjugation with protein

Anglický název

Studying of quantum dot luminescence quenching effect caused by covalent conjugation with protein

Druh

Článek WoS

Originální abstrakt

The characterization of quantum dots (QDs) and their conjugates with bovine serum albumin was done using on-a-chip electrophoresis. Under optimal conditions, bioconjugated QDs were successfully separated from free QDs within approximate to10 min. Influence of coupling agent on a quenching of QD fluorescence intensity caused by conjugation to a protein was studied. When using two-step procedure combining 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide with N-hydroxysulfosuccinimide, the highest quenching effect was notable. We showed that QDs and capillary electrophoresis on a chip can be applied as a sensitive technique for the detection of biological molecules. The characteristics of this method are simplicity, small sample and reagent requirements, and high efficiency of separation.

Anglický abstrakt

The characterization of quantum dots (QDs) and their conjugates with bovine serum albumin was done using on-a-chip electrophoresis. Under optimal conditions, bioconjugated QDs were successfully separated from free QDs within approximate to10 min. Influence of coupling agent on a quenching of QD fluorescence intensity caused by conjugation to a protein was studied. When using two-step procedure combining 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide with N-hydroxysulfosuccinimide, the highest quenching effect was notable. We showed that QDs and capillary electrophoresis on a chip can be applied as a sensitive technique for the detection of biological molecules. The characteristics of this method are simplicity, small sample and reagent requirements, and high efficiency of separation.

Klíčová slova

Bovine serum albumin Bioconjugation Electrophoresis Quantum dots Quenching effect

Klíčová slova v angličtině

Bovine serum albumin Bioconjugation Electrophoresis Quantum dots Quenching effect

Autoři

PEJOVIČ SIMEUNOVIČ, J.; PEKÁRKOVÁ, J.; ŽÁK, J.; CHAMRADOVÁ, I.; HUBÁLEK, J.

Rok RIV

2018

Vydáno

14.07.2017

Nakladatel

Springer

Místo

Vienna

ISSN

1434-4475

Periodikum

MONATSHEFTE FUR CHEMIE

Svazek

148

Číslo

11

Stát

Rakouská republika

Strany od

1901

Strany do

1909

Strany počet

9

URL

https://link.springer.com/content/pdf/10.1007%2Fs00706-017-2038-1.pdf

BibTex

@article{BUT140291,
  author="Jelena {Pejović Simeunović} and Jana {Pekárková} and Jaromír {Žák} and Ivana {Chamradová} and Jaromír {Hubálek}",
  title="Studying of quantum dot luminescence quenching effect caused by covalent conjugation with protein

",
  journal="MONATSHEFTE FUR CHEMIE",
  year="2017",
  volume="148",
  number="11",
  pages="1901--1909",
  doi="10.1007/s00706-017-2038-1",
  issn="0026-9247",
  url="https://link.springer.com/content/pdf/10.1007%2Fs00706-017-2038-1.pdf"
}