Detail publikačního výsledku

Calcium Phosphate Foams: Potential Scaffolds for Bone Tissue Modeling in Three Dimensions

MONTUFAR JIMENEZ, E.; VOJTOVÁ, L.; ČELKO, L.; GINEBRA, M. P.

Originální název

Calcium Phosphate Foams: Potential Scaffolds for Bone Tissue Modeling in Three Dimensions

Anglický název

Calcium Phosphate Foams: Potential Scaffolds for Bone Tissue Modeling in Three Dimensions

Druh

Kapitola, resp. kapitoly v odborné knize

Originální abstrakt

The present method describes the procedure to fabricate calcium phosphate foams with suitable open porosity, pore size, and composition to perform three-dimensional (3D) cell cultures with the objective to simulate the bone tissue microenvironment in vitro. Foams with two compositions but equivalent porosity can be fabricated. On the one hand, hydroxyapatite foams obtained by hydrolysis at 37 °C, with microstructure that mimics the small crystal size of the mineral component of bones, and on the other hand, beta tricalcium phosphate foams with polygonal grains obtained by sintering at 1100 °C. In the first part of the chapter the calcium phosphate foams are briefly described. Afterwards, the foaming process is described in detail, including alternatives to overcome processing problems than can arise. Finally, insights are provided on how to perform 3D cell cultures using the calcium phosphate foams as substrates.

Anglický abstrakt

The present method describes the procedure to fabricate calcium phosphate foams with suitable open porosity, pore size, and composition to perform three-dimensional (3D) cell cultures with the objective to simulate the bone tissue microenvironment in vitro. Foams with two compositions but equivalent porosity can be fabricated. On the one hand, hydroxyapatite foams obtained by hydrolysis at 37 °C, with microstructure that mimics the small crystal size of the mineral component of bones, and on the other hand, beta tricalcium phosphate foams with polygonal grains obtained by sintering at 1100 °C. In the first part of the chapter the calcium phosphate foams are briefly described. Afterwards, the foaming process is described in detail, including alternatives to overcome processing problems than can arise. Finally, insights are provided on how to perform 3D cell cultures using the calcium phosphate foams as substrates.

Klíčová slova

Calcium phosphate foam; Hydroxyapatite; Tricalcium phosphate; Bone model; Cell culture; Three-dimensional; Osteoblast; Tissue engineering; Scaffold

Klíčová slova v angličtině

Calcium phosphate foam; Hydroxyapatite; Tricalcium phosphate; Bone model; Cell culture; Three-dimensional; Osteoblast; Tissue engineering; Scaffold

Autoři

MONTUFAR JIMENEZ, E.; VOJTOVÁ, L.; ČELKO, L.; GINEBRA, M. P.

Rok RIV

2019

Vydáno

21.06.2017

Nakladatel

Springer

ISBN

978-1-4939-7019-3

Kniha

Methods in Molecular Biology

Edice

1612

Strany od

79

Strany do

94

Strany počet

16

URL

https://link.springer.com/protocol/10.1007%2F978-1-4939-7021-6_6

Plný text v Digitální knihovně

http://hdl.handle.net/

BibTex

@inbook{BUT138650,
  author="MONTUFAR JIMENEZ, E. and VOJTOVÁ, L. and ČELKO, L. and GINEBRA, M. P.",
  title="Calcium Phosphate Foams: Potential Scaffolds for Bone Tissue Modeling in Three Dimensions",
  booktitle="Methods in Molecular Biology",
  year="2017",
  publisher="Springer",
  series="1612",
  pages="79--94",
  doi="10.1007/978-1-4939-7021-6\{_}6",
  isbn="978-1-4939-7019-3",
  url="https://link.springer.com/protocol/10.1007%2F978-1-4939-7021-6_6"
}