Detail publikačního výsledku

Label-free bead-based metallothionein electrochemical immunosensor

NEJDL, L.; NGUYEN, H.; RICHTERA, L.; KŘÍŽKOVÁ, S.; GURÁŇ, R.; MASAŘÍK, M.; HYNEK, D.; HEGER, Z.; LUNDBERG, K.; ERIKSON, K.; ADAM, V.; KÍZEK, R.

Originální název

Label-free bead-based metallothionein electrochemical immunosensor

Anglický název

Label-free bead-based metallothionein electrochemical immunosensor

Druh

Článek WoS

Originální abstrakt

A novel microfluidic label-free bead-based metallothionein immunosensors was designed. To the surface of superparamagnetic agarose beads coated with protein A, polyclonal chicken IgY specifically recognizing metallothionein (MT) were immobilized via rabbit IgG. The Brdicka reaction was used for metallothionein detection in a microfluidic printed 3D chip. The assembled chip consisted of a single copper wire coated with a thin layer of amalgam as working electrode. Optimization of MT detection using designed microfluidic chip was performed in stationary system as well as in the flow arrangement at various flow rates (0-1800 L/min). In stationary arrangement it is possible to detect MT concentrations up to 30ng/mL level, flow arrangement allows reliable detection of even lower concentration (12.5ng/mL). The assembled miniature flow chip was subsequently tested for the detection of MT elevated levels (at approx. level 100 g/mL) in samples of patients with cancer. The stability of constructed device for metallothionein detection in flow arrangement was found to be several days without any maintenance needed.

Anglický abstrakt

A novel microfluidic label-free bead-based metallothionein immunosensors was designed. To the surface of superparamagnetic agarose beads coated with protein A, polyclonal chicken IgY specifically recognizing metallothionein (MT) were immobilized via rabbit IgG. The Brdicka reaction was used for metallothionein detection in a microfluidic printed 3D chip. The assembled chip consisted of a single copper wire coated with a thin layer of amalgam as working electrode. Optimization of MT detection using designed microfluidic chip was performed in stationary system as well as in the flow arrangement at various flow rates (0-1800 L/min). In stationary arrangement it is possible to detect MT concentrations up to 30ng/mL level, flow arrangement allows reliable detection of even lower concentration (12.5ng/mL). The assembled miniature flow chip was subsequently tested for the detection of MT elevated levels (at approx. level 100 g/mL) in samples of patients with cancer. The stability of constructed device for metallothionein detection in flow arrangement was found to be several days without any maintenance needed.

Klíčová slova

Immunosensor; Label-free; Metallothionein; Superparamagnetic particles

Klíčová slova v angličtině

Immunosensor; Label-free; Metallothionein; Superparamagnetic particles

Autoři

NEJDL, L.; NGUYEN, H.; RICHTERA, L.; KŘÍŽKOVÁ, S.; GURÁŇ, R.; MASAŘÍK, M.; HYNEK, D.; HEGER, Z.; LUNDBERG, K.; ERIKSON, K.; ADAM, V.; KÍZEK, R.

Rok RIV

2016

Vydáno

01.04.2015

ISSN

0173-0835

Periodikum

ELECTROPHORESIS

Svazek

36

Číslo

16

Stát

Spolková republika Německo

Strany od

1894

Strany do

1904

Strany počet

11

BibTex

@article{BUT123339,
  author="Lukáš {Nejdl} and Kristofer {Erikson} and Vojtěch {Adam} and Hoai Viet {Nguyen} and Lukáš {Richtera} and Soňa {Křížková} and Roman {Guráň} and Michal {Masařík} and David {Hynek} and Zbyněk {Heger} and Karin {Lundberg} and René {Kizek}",
  title="Label-free bead-based metallothionein electrochemical immunosensor",
  journal="ELECTROPHORESIS",
  year="2015",
  volume="36",
  number="16",
  pages="1894--1904",
  doi="10.1002/elps.201500069",
  issn="0173-0835"
}