Detail publikačního výsledku

All-in-one detector of circulating mRNA based on a smartphone

ČMIEL, V.; GUMULEC, J.; SVOBODA, O.; RAUDENSKÁ, M.; HUDCOVÁ, K.; SEKORA, J.; BALOGH, J.; MASAŘÍK, M.; PROVAZNÍK, I.

Originální název

All-in-one detector of circulating mRNA based on a smartphone

Anglický název

All-in-one detector of circulating mRNA based on a smartphone

Druh

Stať ve sborníku v databázi WoS či Scopus

Originální abstrakt

Metallothionein is significantly elevated in various tumors, notably in prostate cancer on both mRNA and protein level. We demonstrated a strong predictive potential of free circulating metallothionein 2A isoform mRNA for patients with this cancer. Circulating mRNA detection relies on expensive equipment and requires high level of expertise. In this work we developed compact "all-in-one" laboratory system which replace microvolume spectrophotometer, thermocycler and realtime PCR machines. We managed to design and construct a microprocessor controlled heating/cooling chamber that ensures required temperature gradient. The chamber includes implemented optical system to enable fluorescence excitation and fluorescence analysis using a smart-phone.

Anglický abstrakt

Metallothionein is significantly elevated in various tumors, notably in prostate cancer on both mRNA and protein level. We demonstrated a strong predictive potential of free circulating metallothionein 2A isoform mRNA for patients with this cancer. Circulating mRNA detection relies on expensive equipment and requires high level of expertise. In this work we developed compact "all-in-one" laboratory system which replace microvolume spectrophotometer, thermocycler and realtime PCR machines. We managed to design and construct a microprocessor controlled heating/cooling chamber that ensures required temperature gradient. The chamber includes implemented optical system to enable fluorescence excitation and fluorescence analysis using a smart-phone.

Klíčová slova

mRNA, smartphone, PCR, cancer, metallothionein

Klíčová slova v angličtině

mRNA, smartphone, PCR, cancer, metallothionein

Autoři

ČMIEL, V.; GUMULEC, J.; SVOBODA, O.; RAUDENSKÁ, M.; HUDCOVÁ, K.; SEKORA, J.; BALOGH, J.; MASAŘÍK, M.; PROVAZNÍK, I.

Rok RIV

2017

Vydáno

04.03.2016

Nakladatel

SPIE

Místo

San Francisco

ISBN

9781628419498

Kniha

Proceedings of SPIE

Strany od

1

Strany do

6

Strany počet

6

URL

http://dx.doi.org/10.1117/12.2213253

BibTex

@inproceedings{BUT122889,
  author="Vratislav {Čmiel} and Jaromír {Gumulec} and Ondřej {Svoboda} and Martina {Raudenská} and Kristýna {Hudcová} and Jiří {Sekora} and Jaroslav {Balogh} and Michal {Masařík} and Valentýna {Provazník}",
  title="All-in-one detector of circulating mRNA based on a smartphone",
  booktitle="Proceedings of SPIE",
  year="2016",
  number="9715",
  pages="1--6",
  publisher="SPIE",
  address="San Francisco",
  doi="10.1117/12.2213253",
  isbn="9781628419498",
  url="http://dx.doi.org/10.1117/12.2213253"
}