Detail publikačního výsledku

Electrochemical Analysis of Metallothionein in Chicken Exposed to Cadmium Ions

TMEJOVÁ, K.; HYNEK, D.; KENŠOVÁ, R.; BLAŽKOVÁ, I.; VYSLOUŽILOVÁ, L.; ŠTĚPÁNKOVÁ, O.; POHANKA, M.; ZEHNÁLEK, J.; VACULOVIČOVÁ, M.; ADAM, V.; KIZEK, R.

Originální název

Electrochemical Analysis of Metallothionein in Chicken Exposed to Cadmium Ions

Anglický název

Electrochemical Analysis of Metallothionein in Chicken Exposed to Cadmium Ions

Druh

Článek WoS

Originální abstrakt

Metallothionein as a protective protein has been studied in relationship to cancer diseases, toxicity of anticancer drugs and environmental pollution. Its electrochemical detection is based on Brdicka´s reaction suitable for the protein detection. The original reaction was invented by Rudolf Brdicka in 1930s and adapted protocol of the reaction was utilized in this study. We focused on the effect of cadmium ions contained in CdTe quantum dots in comparison to cadmium ions in a standard solution applied to chicken embryos. One day after application, chicken embryos were euthanized and liver, kidney, brain and muscle tissue were analyzed. The highest metallothionein levels were detected in the liver and kidney, which corresponded with cadmium accumulation determined by atomic absorption spectroscopy (68 μg/g liver tissue and 125 μg/g kidney tissue after application of quantum dots). The shape of electrochemical voltammograms was mathematically processed. In conclusion, metallothionein seems to be significantly involved in Cd detoxification mechanism.

Anglický abstrakt

Metallothionein as a protective protein has been studied in relationship to cancer diseases, toxicity of anticancer drugs and environmental pollution. Its electrochemical detection is based on Brdicka´s reaction suitable for the protein detection. The original reaction was invented by Rudolf Brdicka in 1930s and adapted protocol of the reaction was utilized in this study. We focused on the effect of cadmium ions contained in CdTe quantum dots in comparison to cadmium ions in a standard solution applied to chicken embryos. One day after application, chicken embryos were euthanized and liver, kidney, brain and muscle tissue were analyzed. The highest metallothionein levels were detected in the liver and kidney, which corresponded with cadmium accumulation determined by atomic absorption spectroscopy (68 μg/g liver tissue and 125 μg/g kidney tissue after application of quantum dots). The shape of electrochemical voltammograms was mathematically processed. In conclusion, metallothionein seems to be significantly involved in Cd detoxification mechanism.

Klíčová slova

Brdicka´s reaction; Cd ions; Metallothionein; Electrochemistry; Toxicity

Klíčová slova v angličtině

Brdicka´s reaction; Cd ions; Metallothionein; Electrochemistry; Toxicity

Autoři

TMEJOVÁ, K.; HYNEK, D.; KENŠOVÁ, R.; BLAŽKOVÁ, I.; VYSLOUŽILOVÁ, L.; ŠTĚPÁNKOVÁ, O.; POHANKA, M.; ZEHNÁLEK, J.; VACULOVIČOVÁ, M.; ADAM, V.; KIZEK, R.

Rok RIV

2016

Vydáno

01.05.2015

Nakladatel

ESG

ISSN

1452-3981

Periodikum

International Journal of Electrochemical Science

Svazek

10

Číslo

5

Stát

Srbská republika

Strany od

3923

Strany do

3934

Strany počet

12

URL

http://www.electrochemsci.org/papers/vol10/100503923.pdf

Plný text v Digitální knihovně

http://hdl.handle.net/11012/201455

BibTex

@article{BUT121159,
  author="Kateřina {Tmejová} and David {Hynek} and Renáta {Kenšová} and Iva {Blažková} and Lenka {Vysloužilová} and Olga {Štěpánková} and Miroslav {Pohanka} and Josef {Zehnálek} and Markéta {Vaculovičová} and Vojtěch {Adam} and René {Kizek}",
  title="Electrochemical Analysis of Metallothionein in Chicken Exposed to Cadmium Ions",
  journal="International Journal of Electrochemical Science",
  year="2015",
  volume="10",
  number="5",
  pages="3923--3934",
  issn="1452-3981",
  url="http://www.electrochemsci.org/papers/vol10/100503923.pdf"
}

Dokumenty

100503923