The adhesion of normal human dermal fibroblasts to the cyclopropylamine plasma polymers studied by holographic microscopy

The adhesion of normal human dermal fibroblasts to the cyclopropylamine plasma polymers studied by holographic microscopy

Článek WoS

The understanding of cell–surface interactions plays an important role for the biomaterials development and bioengineering. Although it is already known that amine groups increase the cell adhesion and proliferation, the influence of amine layers properties on cell viability is the subject of further investigation. In this work, amine-rich coatings were prepared by low pressure plasma polymerization of cyclopropylamine using radio frequency (RF) capacitively coupled discharge. Normal human dermal fibroblasts were chosen for the monitoring of biological response to the properties of amine layers. As a superior technique for the label-free monitoring of the cell–surface interaction, coherence-controlled holographic microscopy (CCHM) was exploited. CCHM enables quantitative phase imaging. From such images, valuable morphological parameters of cells directly related to the cell dry mass can be extracted. Based on those parameters, viability of cells cultivated on the plasmatreated surfaces with different properties was studied and evaluated. According to the results, amine-rich films enhanced the conditions for the cell adhesion and proliferation.

The understanding of cell–surface interactions plays an important role for the biomaterials development and bioengineering. Although it is already known that amine groups increase the cell adhesion and proliferation, the influence of amine layers properties on cell viability is the subject of further investigation. In this work, amine-rich coatings were prepared by low pressure plasma polymerization of cyclopropylamine using radio frequency (RF) capacitively coupled discharge. Normal human dermal fibroblasts were chosen for the monitoring of biological response to the properties of amine layers. As a superior technique for the label-free monitoring of the cell–surface interaction, coherence-controlled holographic microscopy (CCHM) was exploited. CCHM enables quantitative phase imaging. From such images, valuable morphological parameters of cells directly related to the cell dry mass can be extracted. Based on those parameters, viability of cells cultivated on the plasmatreated surfaces with different properties was studied and evaluated. According to the results, amine-rich films enhanced the conditions for the cell adhesion and proliferation.

Plasma polymer; cyclopropylamine; cell adhesion; holographic microscopy; quantitative phase imaging

Plasma polymer; cyclopropylamine; cell adhesion; holographic microscopy; quantitative phase imaging

ŠTRBKOVÁ, L.; MANAKHOV, A.; ZAJÍČKOVÁ, L.; STOICA, A.; VESELÝ, P.; CHMELÍK, R.

2017

15.06.2016

Elsevier B.V.

0257-8972

SURFACE & COATINGS TECHNOLOGY

295

-

Švýcarská konfederace

70

77

8

http://www.sciencedirect.com/science/article/pii/S0257897215303728

http://hdl.handle.net/11012/84148

1-s2.0-S0257897215303728-main