Detail publikačního výsledku

Multimode fibre: Light-sheet microscopy at the tip of a needle

PLÖSCHNER, M.; KOLLÁROVÁ, V.; DOSTÁL, Z.; NYLK, J.; BARTON-OWEN, T.; FERRIER, D.; CHMELÍK, R.; DHOLAKIA, K.; ČIŽMÁR, T.

Originální název

Multimode fibre: Light-sheet microscopy at the tip of a needle

Anglický název

Multimode fibre: Light-sheet microscopy at the tip of a needle

Druh

Článek WoS

Originální abstrakt

Light-sheet fluorescence microscopy has emerged as a powerful platform for 3-D volumetric imaging in the life sciences. Here, we introduce an important step towards its use deep inside biological tissue. Our new technique, based on digital holography, enables delivery of the light-sheet through a multimode optical fibre – an optical element with extremely small footprint, yet permitting complex control of light transport processes within. We show that this approach supports some of the most advanced methods in light-sheet microscopy: by taking advantage of the cylindrical symmetry of the fibre, we facilitate the wavefront engineering methods for generation of both Bessel and structured Bessel beam plane illumination. Finally, we assess the quality of imaging on a sample of fluorescent beads fixed in agarose gel and we conclude with a proof-of-principle imaging of a biological sample, namely the regenerating operculum prongs of Spirobranchus lamarcki.

Anglický abstrakt

Light-sheet fluorescence microscopy has emerged as a powerful platform for 3-D volumetric imaging in the life sciences. Here, we introduce an important step towards its use deep inside biological tissue. Our new technique, based on digital holography, enables delivery of the light-sheet through a multimode optical fibre – an optical element with extremely small footprint, yet permitting complex control of light transport processes within. We show that this approach supports some of the most advanced methods in light-sheet microscopy: by taking advantage of the cylindrical symmetry of the fibre, we facilitate the wavefront engineering methods for generation of both Bessel and structured Bessel beam plane illumination. Finally, we assess the quality of imaging on a sample of fluorescent beads fixed in agarose gel and we conclude with a proof-of-principle imaging of a biological sample, namely the regenerating operculum prongs of Spirobranchus lamarcki.

Klíčová slova

Light-sheet fluorescence microscopy; digital holography

Klíčová slova v angličtině

Light-sheet fluorescence microscopy; digital holography

Autoři

PLÖSCHNER, M.; KOLLÁROVÁ, V.; DOSTÁL, Z.; NYLK, J.; BARTON-OWEN, T.; FERRIER, D.; CHMELÍK, R.; DHOLAKIA, K.; ČIŽMÁR, T.

Rok RIV

2016

Vydáno

14.12.2015

Nakladatel

Springer Nature

ISSN

2045-2322

Periodikum

Scientific Reports

Svazek

5

Číslo

1

Stát

Spojené království Velké Británie a Severního Irska

Strany od

1

Strany do

7

Strany počet

7

URL

https://www.nature.com/articles/srep18050

Plný text v Digitální knihovně

http://hdl.handle.net/11012/179551

BibTex

@article{BUT119568,
  author="Martin {Plöschner} and Věra {Kollárová} and Zbyněk {Dostál} and Jonathan {Nylk} and Thomas {Barton-Owen} and David E.K. {Ferrier} and Radim {Chmelík} and Kishan {Dholakia} and Tomáš {Čižmár}",
  title="Multimode fibre: Light-sheet microscopy at the tip of a needle",
  journal="Scientific Reports",
  year="2015",
  volume="5",
  number="1",
  pages="1--7",
  doi="10.1038/srep18050",
  issn="2045-2322",
  url="https://www.nature.com/articles/srep18050"
}

Dokumenty

srep18050