Detail publikačního výsledku

Surface Passivation Improves the Synthesis of Highly Stable and Specific DNA-Functionalized Gold Nanoparticles with Variable DNA Density

DEKA, J.; MĚCH, R.; IANESELLI, L.; AMENITSCH, H.; CACHO-NERIN, F.; PARISSE, P.; CASALIS, L.

Originální název

Surface Passivation Improves the Synthesis of Highly Stable and Specific DNA-Functionalized Gold Nanoparticles with Variable DNA Density

Anglický název

Surface Passivation Improves the Synthesis of Highly Stable and Specific DNA-Functionalized Gold Nanoparticles with Variable DNA Density

Druh

Článek WoS

Originální abstrakt

We report a novel and multifaceted approach for the quick synthesis of highly stable single-stranded DNA (ssDNA) functionalized gold nanoparticles (AuNPs). The method is based on the combined effect of surface passivation by (1-mercaptoundec-11-yl)hexa(ethylene glycol) and low pH conditions, does not require any salt pretreatment or high excess of ssDNA, and can be generalized for oligonucleotides of any length or base sequence. The synthesized ssDNA-coated AuNPs conjugates are stable at salt concentrations as high as 3.0 M, and also functional and specific toward DNA–DNA hybridization, as shown from UV–vis spectrophotometry, scanning electron microscopy, gel electrophoresis, fluorescence, and small angle X-ray scattering based analyses. The method is highly flexible and shows an additional advantage of creating ssDNA-AuNP conjugates with a predefined number of ssDNA strands per particle. Its simplicity and tenability make it widely applicable to diverse biosensing applications involving ssDNA functionalized AuNPs.

Anglický abstrakt

We report a novel and multifaceted approach for the quick synthesis of highly stable single-stranded DNA (ssDNA) functionalized gold nanoparticles (AuNPs). The method is based on the combined effect of surface passivation by (1-mercaptoundec-11-yl)hexa(ethylene glycol) and low pH conditions, does not require any salt pretreatment or high excess of ssDNA, and can be generalized for oligonucleotides of any length or base sequence. The synthesized ssDNA-coated AuNPs conjugates are stable at salt concentrations as high as 3.0 M, and also functional and specific toward DNA–DNA hybridization, as shown from UV–vis spectrophotometry, scanning electron microscopy, gel electrophoresis, fluorescence, and small angle X-ray scattering based analyses. The method is highly flexible and shows an additional advantage of creating ssDNA-AuNP conjugates with a predefined number of ssDNA strands per particle. Its simplicity and tenability make it widely applicable to diverse biosensing applications involving ssDNA functionalized AuNPs.

Klíčová slova

DNA functionalization; gold nanoparticles; surface passivation; oligo ethylene glycol; salt-stability; specificity; variable DNA density

Klíčová slova v angličtině

DNA functionalization; gold nanoparticles; surface passivation; oligo ethylene glycol; salt-stability; specificity; variable DNA density

Autoři

DEKA, J.; MĚCH, R.; IANESELLI, L.; AMENITSCH, H.; CACHO-NERIN, F.; PARISSE, P.; CASALIS, L.

Rok RIV

2016

Vydáno

10.03.2015

ISSN

1944-8252

Periodikum

ACS Applied Materials & Interfaces

Svazek

7

Číslo

12

Stát

Spojené státy americké

Strany od

7033

Strany do

7040

Strany počet

8

BibTex

@article{BUT114481,
  author="Jashmini {Deka} and Rostislav {Váňa} and Luca {Ianeselli} and Heinz {Amenitsch} and Fernando {Cacho-Nerin} and Pietro {Parisse} and Loredana {Casalis}",
  title="Surface Passivation Improves the Synthesis of Highly Stable and Specific DNA-Functionalized Gold Nanoparticles with Variable DNA Density",
  journal="ACS Applied Materials & Interfaces",
  year="2015",
  volume="7",
  number="12",
  pages="7033--7040",
  doi="10.1021/acsami.5b01191",
  issn="1944-8244"
}