Detail publikačního výsledku

The Electrochemical and Statistical Evaluation of Isolation of Mellitin and Apamin from Honey Bee (Apis Mellifera) Venom

NGUYEN, H.; HEGER, Z.; KOMÍNKOVÁ, M.; MICHÁLEK, P.; GUMULEC, J.; GURÁŇ, R.; PŘIDAL, A.; FERNANDEZ, C.; HYNEK, D.; ADAM, V.; KIZEK, R.

Originální název

The Electrochemical and Statistical Evaluation of Isolation of Mellitin and Apamin from Honey Bee (Apis Mellifera) Venom

Anglický název

The Electrochemical and Statistical Evaluation of Isolation of Mellitin and Apamin from Honey Bee (Apis Mellifera) Venom

Druh

Článek WoS

Originální abstrakt

We present in this manuscript for the first time the electrochemical and statistical evaluation of FPLC isolation of mellitin and apamin from honey bee (Apis mellifera) venom. Venoms are extremely complex blends of diverse substances that target a myriad of receptors or ion channels. Therefore, toxins, isolated from venomous organisms can be a valuable tool with diverse biological applications. In this study we decided to optimize the purification of honey bee venom by using fast protein liquid chromatography, to obtain biologically active peptide - melittin (2846.46 Da). Due to a presence of other compounds with similar molecular weight (apamin 2027.34 Da), we optimized a differential pulse voltammetry method with adsorptive transfer technique (AdT DPV), utilizing Brdicka supporting electrolyte for measurements. Typical voltammograms - fingerprints for each substance were obtained and numerical projections of voltammograms were employed to propose an artificial neural network. Our suggested neural network can simply predict the content of each peptide in fraction with following performance: 100 % for training and 100 % for testing.

Anglický abstrakt

We present in this manuscript for the first time the electrochemical and statistical evaluation of FPLC isolation of mellitin and apamin from honey bee (Apis mellifera) venom. Venoms are extremely complex blends of diverse substances that target a myriad of receptors or ion channels. Therefore, toxins, isolated from venomous organisms can be a valuable tool with diverse biological applications. In this study we decided to optimize the purification of honey bee venom by using fast protein liquid chromatography, to obtain biologically active peptide - melittin (2846.46 Da). Due to a presence of other compounds with similar molecular weight (apamin 2027.34 Da), we optimized a differential pulse voltammetry method with adsorptive transfer technique (AdT DPV), utilizing Brdicka supporting electrolyte for measurements. Typical voltammograms - fingerprints for each substance were obtained and numerical projections of voltammograms were employed to propose an artificial neural network. Our suggested neural network can simply predict the content of each peptide in fraction with following performance: 100 % for training and 100 % for testing.

Klíčová slova

Antimicrobial peptides; Apis mellifera; Brdicka reaction; MALDI-TOF; Differential pulse voltammetry

Klíčová slova v angličtině

Antimicrobial peptides; Apis mellifera; Brdicka reaction; MALDI-TOF; Differential pulse voltammetry

Autoři

NGUYEN, H.; HEGER, Z.; KOMÍNKOVÁ, M.; MICHÁLEK, P.; GUMULEC, J.; GURÁŇ, R.; PŘIDAL, A.; FERNANDEZ, C.; HYNEK, D.; ADAM, V.; KIZEK, R.

Rok RIV

2016

Vydáno

01.02.2015

Nakladatel

ESG

ISSN

1452-3981

Periodikum

International Journal of Electrochemical Science

Svazek

10

Číslo

2

Stát

Srbská republika

Strany od

1249

Strany do

1260

Strany počet

12

URL

http://www.electrochemsci.org/papers/vol10/100201249.pdf

Plný text v Digitální knihovně

http://hdl.handle.net/11012/201450

BibTex

@article{BUT114202,
  author="Hoai Viet {Nguyen} and Zbyněk {Heger} and Markéta {Komínková} and Petr {Michálek} and Jaromír {Gumulec} and Roman {Guráň} and Antonín {Přidal} and Carlos {Fernandez} and David {Hynek} and Vojtěch {Adam} and René {Kizek}",
  title="The Electrochemical and Statistical Evaluation of Isolation of Mellitin and Apamin from Honey Bee (Apis Mellifera) Venom",
  journal="International Journal of Electrochemical Science",
  year="2015",
  volume="10",
  number="2",
  pages="1249--1260",
  issn="1452-3981",
  url="http://www.electrochemsci.org/papers/vol10/100201249.pdf"
}

Dokumenty

100201249