Detail publikačního výsledku

3D-printed chip for detection of methicillin-resistant Staphylococcus aureus labeled with gold nanoparticles

CHUDOBOVÁ, D.; ČÍHALOVÁ, K.; SKALIČKOVÁ, S.; ZÍTKA, O.; MERLOS RODRIGO, M.; MILOSAVLJEVIĆ, V.; HYNEK, D.; KOPEL, P.; VESELÝ, R.; ADAM, V.; KIZEK, R.

Originální název

3D-printed chip for detection of methicillin-resistant Staphylococcus aureus labeled with gold nanoparticles

Anglický název

3D-printed chip for detection of methicillin-resistant Staphylococcus aureus labeled with gold nanoparticles

Druh

Článek WoS

Originální abstrakt

Methicillin-resistant Staphylococcus aureus (MRSA) is a dangerous pathogen occurring not only in hospitals but also in foodstuff. Currently, discussions on the issue of the increasing resistance, and timely and rapid diagnostic of resistance strains have become more frequent and sought. Therefore, the aim of this study was to design an effective platform for DNA isolation from different species ofmicroorganisms as well as the amplification of mecA gene that encodes the resistance to -lactam antibiotic formation and is contained in MRSA. For this purpose, we fabricated 3D-printed chip that was suitable for bacterial cultivation, DNA isolation, PCR, and detection of amplified gene using gold nanoparticle (AuNP) probes as an indicator ofMRSA. Confirmation of the MRSA presence in the samples was based on a specific interaction between mecA gene with the AuNP probes and a colorimetric detection, which utilized the noncross-linking aggregation phenomenon of DNA-functionalized AuNPs.

Anglický abstrakt

Methicillin-resistant Staphylococcus aureus (MRSA) is a dangerous pathogen occurring not only in hospitals but also in foodstuff. Currently, discussions on the issue of the increasing resistance, and timely and rapid diagnostic of resistance strains have become more frequent and sought. Therefore, the aim of this study was to design an effective platform for DNA isolation from different species ofmicroorganisms as well as the amplification of mecA gene that encodes the resistance to -lactam antibiotic formation and is contained in MRSA. For this purpose, we fabricated 3D-printed chip that was suitable for bacterial cultivation, DNA isolation, PCR, and detection of amplified gene using gold nanoparticle (AuNP) probes as an indicator ofMRSA. Confirmation of the MRSA presence in the samples was based on a specific interaction between mecA gene with the AuNP probes and a colorimetric detection, which utilized the noncross-linking aggregation phenomenon of DNA-functionalized AuNPs.

Klíčová slova

3D-printed chip, Gold nanoparticles, Methicillin resistant, Pathogen detection, Staphylococcus aureus

Klíčová slova v angličtině

3D-printed chip, Gold nanoparticles, Methicillin resistant, Pathogen detection, Staphylococcus aureus

Autoři

CHUDOBOVÁ, D.; ČÍHALOVÁ, K.; SKALIČKOVÁ, S.; ZÍTKA, O.; MERLOS RODRIGO, M.; MILOSAVLJEVIĆ, V.; HYNEK, D.; KOPEL, P.; VESELÝ, R.; ADAM, V.; KIZEK, R.

Rok RIV

2016

Vydáno

01.02.2015

ISSN

0173-0835

Periodikum

ELECTROPHORESIS

Svazek

36

Číslo

3

Stát

Spolková republika Německo

Strany od

457

Strany do

466

Strany počet

10

BibTex

@article{BUT113355,
  author="Dagmar {Hegerová} and Kristýna {Číhalová} and Sylvie {Skaličková} and Ondřej {Zítka} and Miguel Ángel {Merlos Rodrigo} and Vedran {Milosavljević} and David {Hynek} and Pavel {Kopel} and Radek {Veselý} and Vojtěch {Adam} and René {Kizek}",
  title="3D-printed chip for detection of methicillin-resistant Staphylococcus aureus labeled with gold nanoparticles",
  journal="ELECTROPHORESIS",
  year="2015",
  volume="36",
  number="3",
  pages="457--466",
  doi="10.1002/elps.201400321",
  issn="0173-0835"
}