Detail publikačního výsledku

Study of metallothionein–quantum dots interactions

TMEJOVÁ, K.; HYNEK, D.; KOPEL, P.; KŘÍŽKOVÁ, S.; TRNKOVÁ, L.; ADAM, V.; KIZEK, R.

Originální název

Study of metallothionein–quantum dots interactions

Anglický název

Study of metallothionein–quantum dots interactions

Druh

Článek WoS

Originální abstrakt

Nanoparticles have gained increasing interest in medical and in vivo applications. Metallothionein (MT)is well known as a maintainer of metal ions balance in intracellular space. This is due to high affinityof this protein to any reactive species including metals and reactive oxygen species. The purpose ofthis study was to determine the metallothionein–quantum dots interactions that were investigated byspectral and electrochemical techniques. CuS, CdS, PbS, and CdTe quantum dots (QDs) were analysed.The highest intensity was shown for CdTe, than for CdS measured by fluorescence. These results weresupported by statistical analysis and considered as significant. Further, these interactions were analysedusing gel electrophoresis, where MT aggregates forming after interactions with QDs were detected. Usingdifferential pulse voltammetry Brdicka reaction, QDs and MT were studied. This method allowed us toconfirm spectral results and, moreover, to observe the changes in MT structure causing new voltammetricpeaks called X and Y, which enhanced with the prolonged time of interaction up to 6 h.

Anglický abstrakt

Nanoparticles have gained increasing interest in medical and in vivo applications. Metallothionein (MT)is well known as a maintainer of metal ions balance in intracellular space. This is due to high affinityof this protein to any reactive species including metals and reactive oxygen species. The purpose ofthis study was to determine the metallothionein–quantum dots interactions that were investigated byspectral and electrochemical techniques. CuS, CdS, PbS, and CdTe quantum dots (QDs) were analysed.The highest intensity was shown for CdTe, than for CdS measured by fluorescence. These results weresupported by statistical analysis and considered as significant. Further, these interactions were analysedusing gel electrophoresis, where MT aggregates forming after interactions with QDs were detected. Usingdifferential pulse voltammetry Brdicka reaction, QDs and MT were studied. This method allowed us toconfirm spectral results and, moreover, to observe the changes in MT structure causing new voltammetricpeaks called X and Y, which enhanced with the prolonged time of interaction up to 6 h.

Klíčová slova

Electrochemical detection, Metallothionein, Quantum dots, Interaction

Klíčová slova v angličtině

Electrochemical detection, Metallothionein, Quantum dots, Interaction

Autoři

TMEJOVÁ, K.; HYNEK, D.; KOPEL, P.; KŘÍŽKOVÁ, S.; TRNKOVÁ, L.; ADAM, V.; KIZEK, R.

Vydáno

01.05.2014

ISSN

0927-7765

Periodikum

COLLOIDS AND SURFACES B-BIOINTERFACES

Svazek

117

Číslo

1

Stát

Nizozemsko

Strany od

534

Strany do

537

Strany počet

4

BibTex

@article{BUT112398,
  author="Kateřina {Tmejová} and David {Hynek} and Pavel {Kopel} and Soňa {Křížková} and Libuše {Trnková} and Vojtěch {Adam} and René {Kizek}",
  title="Study of metallothionein–quantum dots interactions",
  journal="COLLOIDS AND SURFACES B-BIOINTERFACES",
  year="2014",
  volume="117",
  number="1",
  pages="534--537",
  doi="10.1016/j.colsurfb.2014.03.013",
  issn="0927-7765"
}