Detail publikačního výsledku

Application of CdTe/ZnSe Quantum Dots in In Vitro Imaging of Chicken Tissue and Embryo

MOULICK, A.; BLAŽKOVÁ, I.; MILOSAVLJEVIĆ, V.; FOHLEROVÁ, Z.; HUBÁLEK, J.; KOPEL, P.; VACULOVIČOVÁ, M.; ADAM, V.; KIZEK, R.

Originální název

Application of CdTe/ZnSe Quantum Dots in In Vitro Imaging of Chicken Tissue and Embryo

Anglický název

Application of CdTe/ZnSe Quantum Dots in In Vitro Imaging of Chicken Tissue and Embryo

Druh

Článek WoS

Originální abstrakt

The present work is aimed to synthesize CdTe/ZnSe core/ shell quantum dots (QDs) in an easy way and to explore the possibilities of its application in in vitro imaging of chicken tissue and embryo. The QDs were prepared using microwave irradiation with different temperatures, which is a very easy and less time-consuming method. Subsequently, these QDs were characterized by spectrofluorimetry, Transmission Electron Microscopy, X-ray fluorescence analysis and Dynamic Light Scattering measurement. A blueshifting of the emission was found when ZnSe was deposited on CdTe QDs. The QDs showed its fluorescence emission quantum yields up to 25% . They were applied into chicken embryos and breast muscle tissues to study their efficiency in in vitro imaging. All the QDs of different color were able to visualize in in vitro imaging. The highest fluorescence intensity was detected in the case of red QDs prepared at 100 C. The green and red QDs were possible to detect up to the depth of 3 and 4 mm of the tissue, respectively.

Anglický abstrakt

The present work is aimed to synthesize CdTe/ZnSe core/ shell quantum dots (QDs) in an easy way and to explore the possibilities of its application in in vitro imaging of chicken tissue and embryo. The QDs were prepared using microwave irradiation with different temperatures, which is a very easy and less time-consuming method. Subsequently, these QDs were characterized by spectrofluorimetry, Transmission Electron Microscopy, X-ray fluorescence analysis and Dynamic Light Scattering measurement. A blueshifting of the emission was found when ZnSe was deposited on CdTe QDs. The QDs showed its fluorescence emission quantum yields up to 25% . They were applied into chicken embryos and breast muscle tissues to study their efficiency in in vitro imaging. All the QDs of different color were able to visualize in in vitro imaging. The highest fluorescence intensity was detected in the case of red QDs prepared at 100 C. The green and red QDs were possible to detect up to the depth of 3 and 4 mm of the tissue, respectively.

Klíčová slova

NEAR-INFRARED FLUORESCENCE; VIVO; NANOCRYSTALS; NANOPARTICLES; LUMINESCENT; PHOTOLUMINESCENCE; CYTOTOXICITY; EFFICIENCY; STABILITY; GROWTH

Klíčová slova v angličtině

NEAR-INFRARED FLUORESCENCE; VIVO; NANOCRYSTALS; NANOPARTICLES; LUMINESCENT; PHOTOLUMINESCENCE; CYTOTOXICITY; EFFICIENCY; STABILITY; GROWTH

Autoři

MOULICK, A.; BLAŽKOVÁ, I.; MILOSAVLJEVIĆ, V.; FOHLEROVÁ, Z.; HUBÁLEK, J.; KOPEL, P.; VACULOVIČOVÁ, M.; ADAM, V.; KIZEK, R.

Rok RIV

2016

Vydáno

01.04.2015

ISSN

0031-8655

Periodikum

PHOTOCHEMISTRY AND PHOTOBIOLOGY

Svazek

91

Číslo

2

Stát

Spojené státy americké

Strany od

417

Strany do

423

Strany počet

7

BibTex

@article{BUT112156,
  author="Amitava {Moulick} and Iva {Blažková} and Vedran {Milosavljević} and Zdenka {Fohlerová} and Jaromír {Hubálek} and Pavel {Kopel} and Markéta {Vaculovičová} and Vojtěch {Adam} and René {Kizek}",
  title="Application of CdTe/ZnSe Quantum Dots in In Vitro Imaging of Chicken Tissue and Embryo",
  journal="PHOTOCHEMISTRY AND PHOTOBIOLOGY",
  year="2015",
  volume="91",
  number="2",
  pages="417--423",
  doi="10.1111/php.12398",
  issn="0031-8655"
}