Detail publikačního výsledku

Optimisation of DGGE nested PCR for red yeasts characterisation

KOSTOVOVÁ, I.; HÁRONIKOVÁ, A.; PETRIK, S.; MÁROVÁ, I.

Originální název

Optimisation of DGGE nested PCR for red yeasts characterisation

Anglický název

Optimisation of DGGE nested PCR for red yeasts characterisation

Druh

Abstrakt

Originální abstrakt

Carotenogenic yeasts are a diverse group of unrelated organisms (mostly Basidiomycota) that can be found in soil, fresh and marine water, on plants, commonly associated with animals and man-made habitant. In this work the PCR - denaturing gradient gel electrophoresis (DGGE) method was optimized for characterisation of red yeast strains. For this usage, inter-specific variables of strongly conserved sequences of genomic DNA, especially ITS1 and 5.8-ITS2 rDNA regions were amplified. At all, nine of carotenogenic yeast strains of the genus Rhodotorula, Sporobolomyces, Cystofilobasidium and Phaffia were analyzed.

Anglický abstrakt

Carotenogenic yeasts are a diverse group of unrelated organisms (mostly Basidiomycota) that can be found in soil, fresh and marine water, on plants, commonly associated with animals and man-made habitant. In this work the PCR - denaturing gradient gel electrophoresis (DGGE) method was optimized for characterisation of red yeast strains. For this usage, inter-specific variables of strongly conserved sequences of genomic DNA, especially ITS1 and 5.8-ITS2 rDNA regions were amplified. At all, nine of carotenogenic yeast strains of the genus Rhodotorula, Sporobolomyces, Cystofilobasidium and Phaffia were analyzed.

Klíčová slova

DGGE; PCR; red yeast

Klíčová slova v angličtině

DGGE; PCR; red yeast

Autoři

KOSTOVOVÁ, I.; HÁRONIKOVÁ, A.; PETRIK, S.; MÁROVÁ, I.

Vydáno

20.05.2014

Kniha

41st annual conference on yeast

Strany od

55

Strany do

55

Strany počet

86

BibTex

@misc{BUT111756,
  author="Iveta {Kostovová} and Andrea {Němcová} and Siniša {Petrik} and Ivana {Márová}",
  title="Optimisation of DGGE nested PCR for red yeasts characterisation",
  booktitle="41st annual conference on yeast",
  year="2014",
  pages="55--55",
  note="Abstract"
}