Detail publikačního výsledku

Identification of Quantum Dots Labeled Metallothionein by Fast Scanning Laser-Induced Breakdown Spectroscopy

NOVOTNÁ, M.; NOVOTNÝ, K.; KŘÍŽKOVÁ, S.; BLAŽKOVÁ, I.; KOPEL, P.; KAISER, J.; HODEK, P.; KIZEK, R.; ADAM, V.

Originální název

Identification of Quantum Dots Labeled Metallothionein by Fast Scanning Laser-Induced Breakdown Spectroscopy

Anglický název

Identification of Quantum Dots Labeled Metallothionein by Fast Scanning Laser-Induced Breakdown Spectroscopy

Druh

Článek WoS

Originální abstrakt

The technique described in this paper allows detection of quantum dots (QDs) specifically deposited on the polystyrene surface by laser-induced breakdown spectroscopy (LIBS). Using LIBS, the distribution of QDs or their conjugates with biomolecules deposited on the surface can be observed, regardless of the fact if they exhibit fluorescence or not. QDs deposited on the specific surface of polystyrene microplate in the form of spots are detected by determination of the metal included in the QDs structure. Cd-containing QDs (CdS, CdTe) stabilized with mercaptopropionic (MPA) or ercaptosuccinic (MSA) acid, respectively, alone or in the form of conjugates with metallothionein (MT) biomolecule are determined by using the 508.58 nm Cd emission line. The observed absolute detection limit for Cd in CdTe QDs conjugates with MT in one spot was 3 ng Cd. Due to the high sensitivity of this technique, the immunoanalysis in combination with LIBS was also investigated. Cd spatial distribution in sandwich immunoassay was detected.

Anglický abstrakt

The technique described in this paper allows detection of quantum dots (QDs) specifically deposited on the polystyrene surface by laser-induced breakdown spectroscopy (LIBS). Using LIBS, the distribution of QDs or their conjugates with biomolecules deposited on the surface can be observed, regardless of the fact if they exhibit fluorescence or not. QDs deposited on the specific surface of polystyrene microplate in the form of spots are detected by determination of the metal included in the QDs structure. Cd-containing QDs (CdS, CdTe) stabilized with mercaptopropionic (MPA) or ercaptosuccinic (MSA) acid, respectively, alone or in the form of conjugates with metallothionein (MT) biomolecule are determined by using the 508.58 nm Cd emission line. The observed absolute detection limit for Cd in CdTe QDs conjugates with MT in one spot was 3 ng Cd. Due to the high sensitivity of this technique, the immunoanalysis in combination with LIBS was also investigated. Cd spatial distribution in sandwich immunoassay was detected.

Klíčová slova

Laser-induced breakdown spectroscopy; Cadmium; Quantum dots; Metallothionein; Immunoassay

Klíčová slova v angličtině

Laser-induced breakdown spectroscopy; Cadmium; Quantum dots; Metallothionein; Immunoassay

Autoři

NOVOTNÁ, M.; NOVOTNÝ, K.; KŘÍŽKOVÁ, S.; BLAŽKOVÁ, I.; KOPEL, P.; KAISER, J.; HODEK, P.; KIZEK, R.; ADAM, V.

Rok RIV

2017

Vydáno

01.11.2014

ISSN

0584-8547

Periodikum

SPECTROCHIMICA ACTA PART B-ATOMIC SPECTROSCOPY

Svazek

101

Číslo

11

Stát

Spojené království Velké Británie a Severního Irska

Strany od

10

Strany do

16

Strany počet

6

BibTex

@article{BUT109284,
  author="Marie {Novotná} and Karel {Novotný} and Soňa {Křížková} and Iva {Blažková} and Pavel {Kopel} and Jozef {Kaiser} and Petr {Hodek} and René {Kizek} and Vojtěch {Adam}",
  title="Identification of Quantum Dots Labeled Metallothionein by Fast Scanning Laser-Induced Breakdown Spectroscopy",
  journal="SPECTROCHIMICA ACTA PART B-ATOMIC SPECTROSCOPY",
  year="2014",
  volume="101",
  number="11",
  pages="10--16",
  doi="10.1016/j.sab.2014.08.037",
  issn="0584-8547"
}