Detail publikačního výsledku

Paramagnetic particles isolation of influenza oligonucleotide labelled with CdS QDs

KREJČOVÁ, L.; HYNEK, D.; KOPEL, P.; ADAM, V.; HUBÁLEK, J.; TRNKOVÁ, L.; KIZEK, R.

Originální název

Paramagnetic particles isolation of influenza oligonucleotide labelled with CdS QDs

Anglický název

Paramagnetic particles isolation of influenza oligonucleotide labelled with CdS QDs

Druh

Článek WoS

Originální abstrakt

In this study, we describe hybridization design probes consisting of paramagnetic particles and quantum dots (QDs) with targeted DNA, and their application for detection of avian influenza virus (H5N1). Optical properties of QDs were beneficial, but the main attention was paid to the electroactivity of metal part of QDs and ODNs themselves. Differential pulse voltammetry was used for detection of cadmium(II) ions and square wave voltammetry for detection of cytosine–adenine peak in ODN-SH-Cd complex. It clearly follows from the obtained results that the optimized conditions were temperature of hybridization 25 degrees Celsius, time of hybridization 35 min, and concentration of ODN-SH-Cd complex 20 ug mL 1. The detection limit (3 signal/noise) was estimated as 15 ng ml 1 of ODN-SH-Cd.

Anglický abstrakt

In this study, we describe hybridization design probes consisting of paramagnetic particles and quantum dots (QDs) with targeted DNA, and their application for detection of avian influenza virus (H5N1). Optical properties of QDs were beneficial, but the main attention was paid to the electroactivity of metal part of QDs and ODNs themselves. Differential pulse voltammetry was used for detection of cadmium(II) ions and square wave voltammetry for detection of cytosine–adenine peak in ODN-SH-Cd complex. It clearly follows from the obtained results that the optimized conditions were temperature of hybridization 25 degrees Celsius, time of hybridization 35 min, and concentration of ODN-SH-Cd complex 20 ug mL 1. The detection limit (3 signal/noise) was estimated as 15 ng ml 1 of ODN-SH-Cd.

Klíčová slova

paramagnetic particles, DNA, differential pulse voltammetry, square wave voltammetry

Klíčová slova v angličtině

paramagnetic particles, DNA, differential pulse voltammetry, square wave voltammetry

Autoři

KREJČOVÁ, L.; HYNEK, D.; KOPEL, P.; ADAM, V.; HUBÁLEK, J.; TRNKOVÁ, L.; KIZEK, R.

Rok RIV

2014

Vydáno

01.04.2013

ISSN

0009-5893

Periodikum

CHROMATOGRAPHIA

Svazek

76

Číslo

7-8

Stát

Spolková republika Německo

Strany od

355

Strany do

362

Strany počet

8

BibTex

@article{BUT100954,
  author="Ludmila {Krejčová} and David {Hynek} and Pavel {Kopel} and Vojtěch {Adam} and Jaromír {Hubálek} and Libuše {Trnková} and René {Kizek}",
  title="Paramagnetic particles isolation of influenza oligonucleotide labelled with CdS QDs",
  journal="CHROMATOGRAPHIA",
  year="2013",
  volume="76",
  number="7-8",
  pages="355--362",
  doi="10.1007/s10337-012-2327-0",
  issn="0009-5893"
}