Gold Nanoparticles (GNPs) with oxidase activity are prepared and fully characterized. The capture probe for the miR-17-3p a lung cancer biomarker is immobilized on the surface of GNPs that deactivates their oxidase activity. In presence of miR-17-3p the capture probe detaches from the surface of the GNPs which restore their oxidase activity and oxidize the TMB substrate. The oxidized TMB gives electrochemical signal that can be used for the sensitive and selective quantification of miR-17-3p.

CEITEC VUT-J-22-7976

čeština

Garehbaghi Sanam, MEng., M.Sc. - hlavní řešitel
Richtera Lukáš, doc. RNDr., Ph.D. - spoluřešitel

Chytré nanonástroje
- odpovědné pracoviště (3.3.2022 - nezadáno)
Středoevropský technologický institut VUT
- odpovědné pracoviště (8.2.2022 - 3.3.2022)
Středoevropský technologický institut
- interní (1.1.2022 - 31.12.2022)
Chytré nanonástroje
- spolupříjemce (1.3.2022 - 28.2.2023)
Středoevropský technologický institut VUT
- příjemce (1.1.2022 - 31.12.2022)

GAREHBAGHI, S.; ASHRAFI, A.; ADAM, V.; RICHTERA, L. Surface modification strategies and the functional mechanisms of gold nanozyme in biosensing and bioassay. Materials Today Bio, 2023, vol. 20, no. 1, 16 p. ISSN: 2590-0064.
Detail

GAREHBAGHI, S.; RICHTERA, L.; ALTINTAS, Z.; ASHRAFI, A. Tyrosine-Capped Pt Nanozyme Functionalized with cDNA: An Innovative Sensor Designed for One-Step Detection of the miRNA-21 Biomarker. ACS Omega, 2025, vol. 10, no. 35, p. 40324-40334.
Detail

Odpovědnost: Garehbaghi Sanam, MEng., M.Sc.