Gold Nanoparticles (GNPs) with oxidase activity are prepared and fully characterized. The capture probe for the miR-17-3p a lung cancer biomarker is immobilized on the surface of GNPs that deactivates their oxidase activity. In presence of miR-17-3p the capture probe detaches from the surface of the GNPs which restore their oxidase activity and oxidize the TMB substrate. The oxidized TMB gives electrochemical signal that can be used for the sensitive and selective quantification of miR-17-3p.

CEITEC VUT-J-22-7976

Czech

Garehbaghi Sanam, MEng., M.Sc. - principal person responsible
Richtera Lukáš, doc. RNDr., Ph.D. - fellow researcher

Central European Institute of Technology BUT
- responsible department (8.2.2022 - 3.3.2022)
Smart Nanodevices
- responsible department (3.3.2022 - not assigned)
Smart Nanodevices
- co-beneficiary (1.3.2022 - 28.2.2023)
Central European Institute of Technology BUT
- beneficiary (1.1.2022 - 31.12.2022)

GAREHBAGHI, S.; ASHRAFI, A.; ADAM, V.; RICHTERA, L. Surface modification strategies and the functional mechanisms of gold nanozyme in biosensing and bioassay. Materials Today Bio, 2023, vol. 20, no. 1, 16 p. ISSN: 2590-0064.
Detail

GAREHBAGHI, S.; RICHTERA, L.; ALTINTAS, Z.; ASHRAFI, A. Tyrosine-Capped Pt Nanozyme Functionalized with cDNA: An Innovative Sensor Designed for One-Step Detection of the miRNA-21 Biomarker. ACS Omega, 2025, vol. 10, no. 35, p. 40324-40334.
Detail

Responsibility: Garehbaghi Sanam, MEng., M.Sc.