Detail publikačního výsledku

Microfluidic robotic device coupled with electrochemical sensor field for handling of paramagnetic micro-particles as a tool for determination of plant mRNA

HÚSKA, D.; ADAM, V.; BABULA, P.; TRNKOVÁ, L.; HUBÁLEK, J.; ZEHNÁLEK, J.; HAVEL, L.; KIZEK, R.

Originální název

Microfluidic robotic device coupled with electrochemical sensor field for handling of paramagnetic micro-particles as a tool for determination of plant mRNA

Anglický název

Microfluidic robotic device coupled with electrochemical sensor field for handling of paramagnetic micro-particles as a tool for determination of plant mRNA

Druh

Článek recenzovaný mimo WoS a Scopus

Originální abstrakt

The expression of genes responsible for the biosynthesis of stress proteins corresponds to the exposition of an organism to abiotic and/or biotic stress. We utilize two types of paramagnetic particles for isolation of total mRNA from early somatic embryos of Norway Spruce (Picea abies /L./ Karst.) and maize plants (Zea mays L.) treated with cadmium(II) ions. The paramagnetic particles were evaluated for analysis of real samples, and polyadenine was used as a model mRNA. Various approaches (from non-automatic to fully automatic) were tested in terms of handling the particles.

Anglický abstrakt

The expression of genes responsible for the biosynthesis of stress proteins corresponds to the exposition of an organism to abiotic and/or biotic stress. We utilize two types of paramagnetic particles for isolation of total mRNA from early somatic embryos of Norway Spruce (Picea abies /L./ Karst.) and maize plants (Zea mays L.) treated with cadmium(II) ions. The paramagnetic particles were evaluated for analysis of real samples, and polyadenine was used as a model mRNA. Various approaches (from non-automatic to fully automatic) were tested in terms of handling the particles.

Klíčová slova

Electrochemical detection; Microfluidics; Environmental stress; Cadmium ions; Paramagnetic particles

Klíčová slova v angličtině

Electrochemical detection; Microfluidics; Environmental stress; Cadmium ions; Paramagnetic particles

Autoři

HÚSKA, D.; ADAM, V.; BABULA, P.; TRNKOVÁ, L.; HUBÁLEK, J.; ZEHNÁLEK, J.; HAVEL, L.; KIZEK, R.

Rok RIV

2013

Vydáno

01.04.2011

Nakladatel

SPRINGER WIEN,

ISSN

0026-3672

Periodikum

MICROCHIMICA ACTA

Svazek

2012 (173)

Číslo

1-2

Stát

Rakouská republika

Strany od

189

Strany do

197

Strany počet

9

BibTex

@article{BUT75745,
  author="HÚSKA, D. and ADAM, V. and BABULA, P. and TRNKOVÁ, L. and HUBÁLEK, J. and ZEHNÁLEK, J. and HAVEL, L. and KIZEK, R.",
  title="Microfluidic robotic device coupled with electrochemical sensor field for handling of paramagnetic micro-particles as a tool for determination of plant mRNA",
  journal="MICROCHIMICA ACTA",
  year="2011",
  volume="2012 (173)",
  number="1-2",
  pages="189--197",
  issn="0026-3672"
}