Detail publikačního výsledku

Reversible DNA adsorption on the surfaces of different magnetic nano- and microspheres.

ŠPANOVÁ, A.; TRACHTOVÁ, Š.; TVRDÍKOVÁ, J.; HORÁK, D.; RITTICH, B.

Originální název

Reversible DNA adsorption on the surfaces of different magnetic nano- and microspheres.

Anglický název

Reversible DNA adsorption on the surfaces of different magnetic nano- and microspheres.

Druh

Abstrakt

Originální abstrakt

Non-selective isolation of high-quality DNA can be performed using carboxyl group-containing magnetic nonporous hydrophilic poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate) (P(HEMA-co-GMA)) microspheres. This procedure was used for PCR-ready DNA isolation and identification of Lactobacillus, Bifidobacterium and psychrotrophic bacteria in different dairy products or sediments. Analogically the magnetic microparticles P(HEMA-co-GMA) functionalised with streptavidin were used for immobilisation of biotinylated DNA probe and for selective isolation of target DNA from complex samples (e.g. food supplements) using DNA/DNA hybridisation as well as lanthanum manganese perovskites nanoparticles encapsulated into a thin silica shell.

Anglický abstrakt

Non-selective isolation of high-quality DNA can be performed using carboxyl group-containing magnetic nonporous hydrophilic poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate) (P(HEMA-co-GMA)) microspheres. This procedure was used for PCR-ready DNA isolation and identification of Lactobacillus, Bifidobacterium and psychrotrophic bacteria in different dairy products or sediments. Analogically the magnetic microparticles P(HEMA-co-GMA) functionalised with streptavidin were used for immobilisation of biotinylated DNA probe and for selective isolation of target DNA from complex samples (e.g. food supplements) using DNA/DNA hybridisation as well as lanthanum manganese perovskites nanoparticles encapsulated into a thin silica shell.

Klíčová slova

magnetic hydrophilic microspheres; P(HEMA-co-GMA); perovskites nanoparticles; PCR-ready DNA; PCR

Klíčová slova v angličtině

magnetic hydrophilic microspheres; P(HEMA-co-GMA); perovskites nanoparticles; PCR-ready DNA; PCR

Autoři

ŠPANOVÁ, A.; TRACHTOVÁ, Š.; TVRDÍKOVÁ, J.; HORÁK, D.; RITTICH, B.

Rok RIV

2012

Vydáno

28.06.2010

Nakladatel

VUTIUM, Brno

Místo

Brno

ISBN

978-80-214-4112-5

Kniha

In Book of Abstract Sixth International Conference on Materials Structure and Micromechanics of Fracture

Strany od

152

Strany do

152

Strany počet

1

BibTex

@misc{BUT72667,
  author="Alena {Španová} and Štěpánka {Trachtová} and Jana {Tvrdíková} and Daniel {Horák} and Bohuslav {Rittich}",
  title="Reversible DNA adsorption on the surfaces of different magnetic nano- and microspheres.",
  booktitle="In Book of Abstract Sixth International Conference on Materials Structure and Micromechanics of Fracture",
  year="2010",
  pages="152--152",
  publisher="VUTIUM, Brno",
  address="Brno",
  isbn="978-80-214-4112-5",
  note="Abstract"
}